Yanagishita T, Matsuoka T, Kako K J
Department of Physiology, University of Ottawa, School of Medicine, Ontario, Canada.
Biochem Int. 1989 Jun;18(6):1111-9.
Micromolar concentrations of HOCl, an oxidant produced by activated neutrophils, inhibited Ca2+ uptake and Ca2+ATPase of isolated dog heart sarcoplasmic reticulum (SR). DTT antagonized completely the HOCl effect only when it was given within 5 min after the addition of HOCl. When the pharmacological intervention was delayed, the recovery with DTT was not complete, and administration of DTT 30 min after the start of HOCl's reaction with SR resulted in only a small improvement in SR Ca2+ uptake. Although H2O2 and Fe ion-chelate (a free radical-generating procedure) also inhibited Ca2+ uptake and ATPase, the concentrations required were very large. The response of cardiac sarcolemmal and skeletal muscle SR calcium pumps to oxidants was similar to that of the cardiac SR calcium pump.
微摩尔浓度的次氯酸（一种由活化中性粒细胞产生的氧化剂）可抑制离体犬心脏肌浆网（SR）对钙离子的摄取及钙ATP酶活性。仅在添加次氯酸后5分钟内给予二硫苏糖醇（DTT）时，它才能完全拮抗次氯酸的作用。当药理干预延迟时，DTT的恢复并不完全，在次氯酸与SR开始反应30分钟后给予DTT，仅能使SR对钙离子的摄取略有改善。尽管过氧化氢和铁离子螯合物（一种产生自由基的方法）也能抑制钙离子摄取和ATP酶活性，但所需浓度非常高。心肌细胞膜和骨骼肌肌浆网钙泵对氧化剂的反应与心肌肌浆网钙泵相似。
