Rambourg A, Clermont Y, Chrétien M
Département de Biologie du CEA, Saclay, France.
Biol Cell. 1989;65(3):247-56.
The tridimensional structure of the Golgi apparatus has been studied in the absorptive cells of the mouse colon by means of reduced osmium postfixation and phosphatase cytochemistry. In thick sections of tissue impregnated with osmium tetroxide or treated with a technique to demonstrate TPPase activity, the Golgi formed a continuous ribbon-like structure capping the upper pole of the nucleus. Along the longitudinal axis of this ribbon, compact zones made up of superposed flattened saccules alternated with less compact zones which consisted of highly perforated saccules or bridging anastomosed tubules. In the cis-trans axis, the following elements were observed: (1) a cis element consisting of a continuous osmiophilic tubular network; (2) two or three subjacent elements selectively perforated by wells; (3) a trans compartment made up of two or three TPPase-reactive sacculotubular elements, some showing a "peeling-off" configuration. In some regions, the first flattened saccule of this trans compartment displayed discrete ovoid dilatations, located in compact zones and containing a dense granulofibrillar material; in the subjacent elements this material was seen concentrated in nodular swellings, at the intersection of the meshes of anastomosed membranous tubules. 100-300 nm vesicles containing a similar dense granulofilamentous material were observed in the trans Golgi zone and interspersed in the supranuclear cytoplasm between the Golgi zone and the apical surface of the cell. Smaller vesicles 80-100 nm in diameter containing a fine dusty material were also seen in proximity. These morphological observations suggested that at least two kinds of material were segregated in the saccules of the trans compartment and packaged in vesicles of two class sizes that detached from the Golgi stack on its trans aspect.
通过还原锇后固定和磷酸酶细胞化学方法，对小鼠结肠吸收细胞中高尔基体的三维结构进行了研究。在用四氧化锇浸渍的组织厚切片中，或用显示TPPase活性的技术处理的组织厚切片中，高尔基体形成连续的带状结构，覆盖在细胞核的上极。沿着这条带的纵轴，由叠加的扁平囊泡组成的致密区与由高度穿孔的囊泡或桥接吻合小管组成的不太致密的区交替出现。在顺-反轴上，观察到以下成分：(1) 由连续的嗜锇管状网络组成的顺面成分；(2) 两到三个被孔选择性穿孔的相邻成分；(3) 由两到三个TPPase反应性囊管状成分组成的反面区室，一些呈现“剥离”构型。在某些区域，这个反面区室的第一个扁平囊泡显示出离散的卵圆形扩张，位于致密区，含有致密的颗粒纤维状物质；在相邻成分中，这种物质集中在结节状肿胀处，位于吻合的膜性小管网的交叉处。在反面高尔基体区观察到含有类似致密颗粒丝状物质的100 - 300 nm囊泡，并散布在高尔基体区和细胞顶端表面之间的核上细胞质中。在附近还可见到直径80 - 100 nm、含有细尘状物质的较小囊泡。这些形态学观察表明，至少两种物质在反面区室的囊泡中被分离，并被包装在两种大小的囊泡中，这些囊泡从高尔基体堆叠的反面脱离。
