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Tad转座子在脉孢菌菌株中的分布受限。

Restricted distribution of the Tad transposon in strains of Neurospora.

作者信息

Kinsey J A

机构信息

Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66103.

出版信息

Curr Genet. 1989 Apr;15(4):271-5. doi: 10.1007/BF00447042.

DOI:10.1007/BF00447042
PMID:2546685
Abstract

A simple colony blot procedure was used to screen 336 Neurospora strains for the presence of the transposable element Tad. These strains included the standard laboratory wild types, all of the available Neurospora isolates collected from the Ivory Coast, and all wild-collected Neurospora crassa isolates available from the Fungal Genetics Stock Center. Tad was found only in the strain of origin from Adiopodumé, Ivory Coast, where it is present in multiple copies. Three other strains of African origin were found to have single copy sequences that are related to Tad.

摘要

使用一种简单的菌落杂交程序，对336株脉孢菌菌株进行筛选，以检测转座元件Tad的存在。这些菌株包括标准实验室野生型、从象牙海岸收集的所有可用脉孢菌分离株，以及从真菌遗传种质中心获得的所有野生采集的粗糙脉孢菌分离株。仅在来自象牙海岸阿迪奥波杜梅的原始菌株中发现了Tad，该菌株中存在多个拷贝。另外三株非洲起源的菌株被发现具有与Tad相关的单拷贝序列。

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