一种用于丝状真菌粗糙脉孢菌的菌落滤膜杂交程序。
A colony filter-hybridization procedure for the filamentous fungus Neurospora crassa.
作者信息
Stohl L L, Lambowitz A M
出版信息
Anal Biochem. 1983 Oct 1;134(1):82-5. doi: 10.1016/0003-2697(83)90266-x.
PMID:6229196
Abstract
A colony filter-hybridization procedure for the filamentous fungus Neurospora crassa has been developed. The procedure is sensitive enough to detect Escherichia coli plasmid pBR322 DNA integrated into chromosomal DNA in a Neurospora transformant. Thus, it should facilitate the isolation of nuclear genes by plasmid-rescue procedures.
摘要
已开发出一种用于丝状真菌粗糙脉孢菌的菌落滤膜杂交程序。该程序灵敏度足以检测整合到脉孢菌转化体染色体DNA中的大肠杆菌质粒pBR322 DNA。因此，它应有助于通过质粒拯救程序分离核基因。
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