Previato M, Borim M P, Liberatore R D R, Pires A C, Dias M A F, Brandão de Mattos C C, de Mattos L C
Immunogenetics Laboratory, Department of Molecular Biology, Faculdade de Medicina de São José do Rio Preto, FAMERP, São José do Rio Preto, Brazil.
Vox Sang. 2015 Apr;108(3):281-6. doi: 10.1111/vox.12211. Epub 2014 Dec 3.
The red blood cell Le(a-b-) phenotype was proposed as risk factor for type 1 diabetes, but contradictory results were published elsewhere. This study re-examined the potential association between Lewis histo-blood group system and type 1 diabetes.
Patients and controls of both sexes, Caucasians and non-Caucasians, matched by sex, geographical origin and ethnicity were evaluated. The red blood cell Lewis phenotypes were identified by gel column agglutination and also inferred from the FUT2 and FUT3 genotyping.
The Le(a-b-) phenotype was prevalent in patients with type 1 diabetes, and the Le(a-b+) phenotype was prevalent in controls when both were determined by gel columns agglutination. No differences were observed in the frequencies of the Le(a-b-) phenotype inferred from the FUT2 and FUT3 genotyping between patients and controls.
The Lewis red blood cell phenotyping and genotyping reveal divergence in the association of Le(a-b-) phenotype and type 1 diabetes.
红细胞Le(a-b-)表型被认为是1型糖尿病的危险因素，但其他地方发表了相互矛盾的结果。本研究重新审视了Lewis组织血型系统与1型糖尿病之间的潜在关联。
对按性别、地理来源和种族匹配的男性和女性、白种人和非白种人的患者及对照进行评估。通过凝胶柱凝集鉴定红细胞Lewis表型，并从FUT2和FUT3基因分型推断。
当通过凝胶柱凝集确定时，Le(a-b-)表型在1型糖尿病患者中普遍存在，而Le(a-b+)表型在对照中普遍存在。患者和对照之间从FUT2和FUT3基因分型推断的Le(a-b-)表型频率没有差异。
Lewis红细胞表型分析和基因分型揭示了Le(a-b-)表型与1型糖尿病关联的差异。
