Nakashima Fabiana, Brandão de Mattos Cinara Cássia, Ferreira Ana Iara Costa, Spergiorin Lígia Cosentino Junqueira Franco, Meira-Strejevitch Cristina Silva, Oliani Antonio Hélio, Vaz-Oliani Denise Cristina Mós, Pereira-Chioccola Vera Lúcia, de Mattos Luiz Carlos
Biology Department, Bioscience, Languages and Exact Sciences Institute of the Universidade Estadual Paulista "Júlio de Mesquita Filho" (IBILCE/UNESP), São José do Rio Preto, São Paulo, Brazil; Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, São Paulo, Brazil.
Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, São Paulo, Brazil; FAMERP Toxoplasma Research Group, Brazil.
Acta Trop. 2019 May;193:92-98. doi: 10.1016/j.actatropica.2019.02.031. Epub 2019 Mar 1.
The interaction between the ABO, FUT2 and FUT3 genes results in the synthesis of different glycoconjugates profiles expressed in gastrointestinal tract. Moreover, the protozoan Toxoplasma gondii, which causes toxoplasmosis, utilizes this organ as an infection route. We analyzed the frequencies of the different glycoconjugate profiles which were determined by phenotyping ABO and genotyping the status secretor (FUT2; substitution G428A) and Lewis (FUT3; substitution T202C and C314T) histo-blood systems, assessed by PCR-RFLP and PCR-SSP, respectively. A total of 244 pregnant women (G1: Seropositive; G2: Seronegative) for IgG T. gondii antibodies were enrolled. IgG anti-T. gondii antibodies were determined by ELISA. G1 was composed of 158 (64.8%) sample and G2 by 86 (36.2%). The glycoconjugate profile was accessed in 151 seropositive and 85 seronegative samples by the combination of ABO and Lewis phenotyping as well as FUT2 and FUT3 genotyping. In G1, 36 (22.8%) presented the glycoconjugate profile ALe, 5 (3.3%) A, 13 (8.6) BLe, 1 (0.6%) B, 41 (27.1%) Le, 13(8.6%) H, 38(25.2%) Le and 4 (2.6%) Le. G2 was composed of 13 (15.3%) of ALe, 15 (17.6%) BLe, 1 (1.2%) B, 42 (49,4%) Le and 14 (16.5) Le. H and Le glycoconjugate profiles were not found in G2. The frequencies of the glycoconjugates profiles Le (p = 0.001) and H (p = 0.005) were significantly different compared between G1 and G2. The glycoconjugate profile H inferred from the ABO phenotyping and FUT3 and FUT2 genotyping is associated with infection by T. gondii in pregnant women and the Le profile appears to protect the infection by this parasite.
ABO、FUT2和FUT3基因之间的相互作用导致胃肠道中表达不同的糖缀合物谱。此外，引起弓形虫病的原生动物刚地弓形虫利用该器官作为感染途径。我们分析了通过ABO表型分型以及分泌型（FUT2；G428A替换）和Lewis（FUT3；T202C和C314T替换）组织血型系统基因分型确定的不同糖缀合物谱的频率，分别通过PCR-RFLP和PCR-SSP进行评估。共纳入244名弓形虫IgG抗体检测呈阳性（G1组）和阴性（G2组）的孕妇。通过ELISA检测弓形虫IgG抗体。G1组由158个样本（64.8%）组成，G2组由86个样本（36.2%）组成。通过ABO和Lewis表型分型以及FUT2和FUT3基因分型的组合，对151份血清阳性样本和85份血清阴性样本进行了糖缀合物谱分析。在G1组中，36份（22.8%）呈现糖缀合物谱ALe，5份（3.3%）为A，13份（8.6%）为BLe，1份（0.6%）为B，41份（27.1%）为Le，13份（8.6%）为H，38份（25.2%）为Le，4份（2.6%）为Le。G2组由13份（15.3%）的ALe、15份（17.6%）的BLe、1份（1.2%）的B、42份（49.4%）的Le和14份（16.5%）的Le组成。G2组未发现H和Le糖缀合物谱。G1组和G2组之间，糖缀合物谱Le（p = 0.001）和H（p = 0.005）的频率存在显著差异。通过ABO表型分型以及FUT3和FUT2基因分型推断出的糖缀合物谱H与孕妇感染刚地弓形虫有关，而Le谱似乎对这种寄生虫的感染具有保护作用。
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