Ameno S, Kimura H, Ameno K, Zhang X, Kinoshita H, Kubota T, Ijiri I
Department of Forensic Medicine, Faculty of Medicine, Kagawa Medical University, Kagawa, Japan.
Biol Neonate. 2001 Feb;79(2):91-6. doi: 10.1159/000047073.
Lewis (Le) and Secretor (Se) gene effects on postnatal change in Lewis antigens are described based on the quantitative analysis of Lewis antigens in blood. Genuine Lewis blood types were determined by Le and Se genotyping in DNA samples obtained from umbilical cord blood and infant blood. Lewis(a) (Le(a)) and Lewis(b) (Le(b)) antigen levels were measured by a time-resolved fluoroimmunoassay. We found that Lewis phenotypes in infants over 9 months of age agreed with the genuine types. In cord blood, the dosage effect of the Se gene on antigen levels was observed, but the Le gene effect was not observed. Both Se and Le gene effects were observed in newborn babies' blood 3-6 days after birth.
基于血液中Lewis抗原的定量分析，描述了Lewis（Le）和分泌型（Se）基因对出生后Lewis抗原变化的影响。通过对从脐带血和婴儿血液中获取的DNA样本进行Le和Se基因分型，确定真正的Lewis血型。采用时间分辨荧光免疫测定法测量Lewis(a)（Le(a)）和Lewis(b)（Le(b)）抗原水平。我们发现，9个月以上婴儿的Lewis表型与真正的血型一致。在脐带血中，观察到Se基因对抗原水平的剂量效应，但未观察到Le基因效应。在出生后3 - 6天的新生儿血液中，观察到了Se和Le基因的效应。
