Bergmann U, Michaelis J, Oberhoff R, Knäuper V, Beckmann R, Tschesche H
Fakultät für Chemie, Lehrstuhl Biochemie, Universität Bielefeld.
J Clin Chem Clin Biochem. 1989 Jun;27(6):351-9. doi: 10.1515/cclm.1989.27.6.351.
A competitive and a sandwich enzyme linked immunosorbent assay (ELISA) were developed for human leukocyte collagenase and gelatinase. The competitive assay could detect 0.5 ng collagenase and 0.05 ng gelatinase. The detection limit of the sandwich ELISA was 0.05 ng for collagenase and 0.02 ng for gelatinase. No cross reactivity between human leukocyte collagenase and gelatinase was detected. The sandwich ELISA was used to determine plasma levels of these enzymes. The 90% range for collagenase was between 0 and 50 micrograms/l; the 90% range for gelatinase was between 27 and 94 micrograms/l.
开发了一种用于检测人白细胞胶原酶和明胶酶的竞争性和夹心酶联免疫吸附测定（ELISA）。竞争性测定法可检测到0.5纳克胶原酶和0.05纳克明胶酶。夹心ELISA法对胶原酶的检测限为0.05纳克，对明胶酶的检测限为0.02纳克。未检测到人白细胞胶原酶和明胶酶之间的交叉反应。夹心ELISA法用于测定这些酶的血浆水平。胶原酶的90%范围在0至50微克/升之间；明胶酶的90%范围在27至94微克/升之间。
