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由设计的转录激活样效应因子（TALE）诱导的抗病毒β-干扰素信号传导

Antiviral interferon-beta signaling induced by designed transcription activator-like effectors (TALE).

作者信息

Renner Ivonne, Funk Nancy, Geissler Rene, Friedrich Susann, Penzel Anika, Behrens Sven-Erik

机构信息

Institute of Biochemistry and Biotechnology, Section Microbial Biotechnology, Martin Luther University Halle-Wittenberg, Faculty of Life Sciences (NFI), Kurt-Mothes-Str. 3, D-06120, Halle/Saale, Germany.

出版信息

PLoS One. 2014 Dec 3;9(12):e114288. doi: 10.1371/journal.pone.0114288. eCollection 2014.

DOI:10.1371/journal.pone.0114288
PMID:25470486
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4255017/
Abstract

Here we show that designed transcription activator-like effectors (TALEs) that bind to defined areas of the interferon beta promoter are capable to induce IFN-beta expression and signaling in human cells. Importantly, TALE-mediated IFN-beta signaling occurs independently of pathogen pattern recognition but effectively prohibits viral RNA replication as demonstrated with a hepatitis C virus replicon. TALEs were thus indicated to be valuable tools in various applications addressing, for example, virus-host interactions.

摘要

在此我们表明，设计的与干扰素β启动子特定区域结合的转录激活样效应因子（TALEs）能够在人类细胞中诱导IFN-β表达和信号传导。重要的是，TALE介导的IFN-β信号传导独立于病原体模式识别发生，但如丙型肝炎病毒复制子所示，能有效阻止病毒RNA复制。因此，TALEs被认为是在各种应用中，例如解决病毒-宿主相互作用方面有价值的工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/576e/4255017/c2a4010b43fe/pone.0114288.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/576e/4255017/e91395022add/pone.0114288.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/576e/4255017/c2a4010b43fe/pone.0114288.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/576e/4255017/e91395022add/pone.0114288.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/576e/4255017/c2a4010b43fe/pone.0114288.g002.jpg

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本文引用的文献

1
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Trends Cell Biol. 2013 Aug;23(8):390-8. doi: 10.1016/j.tcb.2013.04.003. Epub 2013 May 23.
2
Innate immune responses in hepatitis C virus infection.丙型肝炎病毒感染中的固有免疫应答。
Semin Immunopathol. 2013 Jan;35(1):53-72. doi: 10.1007/s00281-012-0332-x. Epub 2012 Aug 7.
3
Interferon-inducible effector mechanisms in cell-autonomous immunity.
细胞自主免疫中的干扰素诱导效应机制。
Nat Rev Immunol. 2012 Apr 25;12(5):367-82. doi: 10.1038/nri3210.
4
Induction and function of type I and III interferon in response to viral infection.诱导和 I 型和 III 型干扰素在病毒感染中的功能。
Curr Opin Virol. 2011 Dec;1(6):476-86. doi: 10.1016/j.coviro.2011.11.001.
5
The DEAD-box helicase DDX3 supports the assembly of functional 80S ribosomes.DEAD-box 解旋酶 DDX3 支持功能性 80S 核糖体的组装。
Nucleic Acids Res. 2012 Jun;40(11):4998-5011. doi: 10.1093/nar/gks070. Epub 2012 Feb 9.
6
Transcriptional activators of human genes with programmable DNA-specificity.具有可编程 DNA 特异性的人类基因转录激活剂。
PLoS One. 2011;6(5):e19509. doi: 10.1371/journal.pone.0019509. Epub 2011 May 19.
7
A diverse range of gene products are effectors of the type I interferon antiviral response.多种基因产物是 I 型干扰素抗病毒反应的效应物。
Nature. 2011 Apr 28;472(7344):481-5. doi: 10.1038/nature09907. Epub 2011 Apr 10.
8
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9
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Nat Biotechnol. 2011 Feb;29(2):143-8. doi: 10.1038/nbt.1755. Epub 2010 Dec 22.
10
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