Watkins B A
Department of Poultry Science, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Poult Sci. 1989 May;68(5):698-705. doi: 10.3382/ps.0680698.
Male broiler chicks were fed a purified diet with 0 or 500 micrograms/kg of added biotin for 21 days posthatch. Fatty acid composition of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were determined in erythrocytes and in heart mitochondria and microsomes of chicks at 12 and 21 days of age. The biotin-deficient (BD, 0 microgram biotin) chicks had lower body weights, lower feed efficiencies, and higher scores for dermatitis and twisted leg than the biotin-adequate (BA, 500 micrograms biotin) chicks. The erythrocyte arachidonate level in PC was higher in BD chicks compared to the BA controls, and the level increased with age in PC and PE in the erythrocytes. The level of dihomo-gamma-linolenate (20:3 omega 6) in PC and PE in heart mitochondria and microsomes was consistently decreased in BD chicks compared with that in BA chicks. In heart subcellular organelles, the level of arachidonate in PC increased, but the level of dihomo-gamma-linolenate in PE decreased with age. These data demonstrate that biotin deficiency results in dramatic changes in the fatty acid composition of specific phospholipids in chick erythrocytes and heart subcellular organelles.
雄性肉仔鸡在出壳后21天内被饲喂添加了0或500微克/千克生物素的纯化日粮。在12日龄和21日龄时，测定了雏鸡红细胞、心脏线粒体和微粒体中磷脂酰胆碱（PC）和磷脂酰乙醇胺（PE）的脂肪酸组成。与生物素充足（BA，500微克生物素）的雏鸡相比，生物素缺乏（BD，0微克生物素）的雏鸡体重更低、饲料效率更低，且皮炎和腿部扭曲的评分更高。与BA对照组相比，BD雏鸡红细胞中PC的花生四烯酸水平更高，且红细胞中PC和PE的该水平随年龄增加。与BA雏鸡相比，BD雏鸡心脏线粒体和微粒体中PC和PE的二高-γ-亚麻酸（20:3ω6）水平持续降低。在心脏亚细胞器中，PC中的花生四烯酸水平升高，但PE中的二高-γ-亚麻酸水平随年龄降低。这些数据表明，生物素缺乏会导致雏鸡红细胞和心脏亚细胞器中特定磷脂的脂肪酸组成发生显著变化。
