Gao Bingbing, Liu Hong, Gu Zhongze
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University , Nanjing 210096, China.
Langmuir. 2014 Dec 23;30(50):15041-6. doi: 10.1021/la503720b. Epub 2014 Dec 8.
We report a method for the bottom-up fabrication of paper-based capillary microchips by the blade coating of cellulose microfibers on a patterned surface. The fabrication process is similar to the paper-making process in which an aqueous suspension of cellulose microfibers is used as the starting material and is blade-coated onto a polypropylene substrate patterned using an inkjet printer. After water evaporation, the cellulose microfibers form a porous, hydrophilic, paperlike pattern that wicks aqueous solution by capillary action. This method enables simple, fast, inexpensive fabrication of paper-based capillary channels with both width and height down to about 10 μm. When this method is used, the capillary microfluidic chip for the colorimetric detection of glucose and total protein is fabricated, and the assay requires only 0.30 μL of sample, which is 240 times smaller than for paper devices fabricated using photolithography.
我们报道了一种通过在图案化表面上刮涂纤维素微纤维自下而上制备纸基毛细管微芯片的方法。制造过程类似于造纸过程，其中纤维素微纤维的水悬浮液用作起始材料，并刮涂到使用喷墨打印机图案化的聚丙烯基板上。水蒸发后，纤维素微纤维形成多孔、亲水、类似纸的图案，通过毛细管作用吸收水溶液。该方法能够简单、快速、廉价地制造宽度和高度均低至约10μm的纸基毛细管通道。使用该方法时，制造了用于比色法检测葡萄糖和总蛋白的毛细管微流控芯片，该检测仅需要0.30μL样品，这比使用光刻法制造的纸质设备所需样品量小240倍。
