Wehmann Enikő, Khayer Bernadett, Magyar Tibor
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, 1143, Budapest, Hungary,
Arch Microbiol. 2015 Jan;197(1):105-12. doi: 10.1007/s00203-014-1068-x. Epub 2014 Dec 5.
Bordetella bronchiseptica is a widespread pathogen, with a broad host range, occasionally including humans. Diverse virulence factors (adhesins, toxins) allow its adaptation to its host, but this property of the adenylate cyclase (cyaA) toxin is not well understood. In this study, we analyzed the repeats-in-toxin domain of B. bronchiseptica cyaA with PCR, followed by restriction fragment length analysis. Of ninety-two B. bronchiseptica strains collected from different hosts and geographic regions, 72 (78.3 %) carried cyaA and four RFLP types (A-D) were established using NarI and SalI. However, in 20 strains, cyaA was replaced with a peptide transport protein operon. A phylogenetic tree based on partial nucleotide sequences of cyaA revealed that group 2 contains strains of specifically human origin, whereas subgroup 1a contains all but one of the strains from pigs. The human strains showed many PCR-RFLP and sequence variants, confirming the clonal population structure of B. bronchiseptica.
支气管败血波氏杆菌是一种广泛传播的病原体，宿主范围广泛，偶尔也包括人类。多种毒力因子（黏附素、毒素）使其能够适应宿主，但其腺苷酸环化酶（cyaA）毒素的这一特性尚未得到充分了解。在本研究中，我们通过聚合酶链反应（PCR）分析了支气管败血波氏杆菌cyaA的毒素重复结构域，随后进行了限制性片段长度分析。在从不同宿主和地理区域收集的92株支气管败血波氏杆菌菌株中，72株（78.3%）携带cyaA，使用NarI和SalI确定了四种限制性片段长度多态性（RFLP）类型（A - D）。然而，在20株菌株中，cyaA被肽转运蛋白操纵子所取代。基于cyaA部分核苷酸序列构建的系统发育树显示，第2组包含特定人类来源的菌株，而第1a亚组包含除一株以外的所有猪源菌株。人类菌株表现出许多PCR - RFLP和序列变异，证实了支气管败血波氏杆菌的克隆群体结构。
