Tanniou Anaëlle, Vandanjon Laurent, Gonçalves Olivier, Kervarec Nelly, Stiger-Pouvreau Valérie
LEMAR UMR CNRS UBO IRD IFREMER 6539, Université de Bretagne Occidentale (UBO), Institut Universitaire Européen de la Mer (IUEM), Technopôle Brest-Iroise, Rue Dumont d׳Urville, Plouzané 29280, France.
GEPEA UMR CNRS 6144, Université de Nantes, Laboratoire GEPEA, CRTT, 44602 Saint-Nazaire, France; LBCM EA 3884, Université de Bretagne Sud (UBS), IUEM, Campus de Tohannic, 56000 Vannes, France.
Talanta. 2015 Jan;132:451-6. doi: 10.1016/j.talanta.2014.09.002. Epub 2014 Sep 22.
Two recent techniques based on chemical footprinting analysis, HRMAS NMR and FTIR spectroscopy, were tested on a brown macroalgal model. These powerful and easily-to-use techniques allowed us to discriminate Sargassum muticum specimens collected in five different countries along Atlantic coasts, from Portugal to Norway. HRMAS NMR and FTIR permitted the obtaining of an overview of metabolites produced by the alga. Based on spectra analysis, results allowed us to successfully group the samples according to their geographical origin. HRMAS NMR and FTIR spectroscopy respectively point out the relation between the geographical localization and the chemical composition and demonstrated macromolecules variations regarding to environmental stress. Then, our results are discussed in regard of the powerful of these techniques together with the variability of the main molecules produced by Sargassum muticum along the Atlantic coasts.
基于化学足迹分析的两种最新技术，即高分辨魔角旋转核磁共振（HRMAS NMR）和傅里叶变换红外光谱（FTIR），在一种棕色大型海藻模型上进行了测试。这些强大且易于使用的技术使我们能够区分沿着大西洋海岸从葡萄牙到挪威的五个不同国家采集的马尾藻标本。HRMAS NMR和FTIR能够让我们对该海藻产生的代谢物有一个总体了解。基于光谱分析，结果使我们能够根据样品的地理来源成功地对其进行分组。HRMAS NMR和FTIR光谱分别指出了地理定位与化学成分之间的关系，并证明了在环境压力下大分子的变化。然后，我们结合这些技术的强大功能以及大西洋沿岸马尾藻产生的主要分子的变异性来讨论我们的结果。
