Department of Biochemistry, University of Kashmir, Srinagar-190006, J&K, India.
Indian Institute of Integrative Medicine, Srinagar, India.
J Ethnopharmacol. 2015 Mar 13;162:215-24. doi: 10.1016/j.jep.2014.08.008. Epub 2014 Dec 2.
Atropa acuminata has been widely used in traditional medicine against arthritis and several associated inflammatory disorders.
The present study was undertaken to investigate the anti-arthritic activities of ethanolic extract of Atropa accuminata (AAEE) and to explore the probable mechanism of action.
The anti-arthritic activity of AAEE was evaluated within a dose range of 125-500 mg/kg b.w. in adjuvant induced-arthritis in male wistar rats. An array of pro-inflammatory mediators (PGE2 NO, IL-1β and LTB4) and T-cell-mediated cytokines (IL-2, TNF-a, IFN-c, IL-4, IL-10, IL-12, IL-17, IL-6) were assayed in arthritic paw tissue homogenate of the treated animals. In addition the effects on arthritic lesions, changes in body weight; haematological (Hb, ESR, WBC and RBC) and biochemical parameters (SOD, GSH, GR) and the serum markers (CRP, RF) were also observed.
Significant anti-arthritic activity was observed for AAEE in the polyarthiritis test both in the developing and developed phase of the disease. This was associated with dose dependant suppression of pro-inflammatory mediators (PGE2, NO, IL-1β and LTB4)., Th1-Th17 cytokines (IL-2, TNF-α, IFN-γ, IL-12, IL-17, IL-6) and upregulation of Th2 cytokines (IL-4 and IL-10). AAEE was also observed to protect rats against the primary and secondary arthritic lesions, body weight changes and haematological perturbations. In addition, inhibitory effects of AAEE on biochemical parameters and the serum markers further confirmed that it reduced signs on chronic inflammatory responses.
The present investigation therefore suggested that AAEE is a potent anti-arthritic agent. The multipronged attack on the inflammatory mediators and T-helper cytokines and strong potency of AAEE may have relevance for inhibition of the chronic inflammatory responses in arthritis.
颠茄已被广泛用于传统医学治疗关节炎和几种相关的炎症性疾病。
本研究旨在研究颠茄乙醇提取物（AAEE）的抗关节炎活性，并探讨其可能的作用机制。
在雄性 Wistar 大鼠佐剂诱导关节炎模型中，评价 AAEE 的抗关节炎活性，剂量范围为 125-500mg/kgbw。在治疗动物的关节炎爪组织匀浆中测定一系列促炎介质（PGE2、NO、IL-1β 和 LTB4）和 T 细胞介导的细胞因子（IL-2、TNF-α、IFN-c、IL-4、IL-10、IL-12、IL-17、IL-6）。此外，还观察了对关节炎病变、体重变化、血液学（Hb、ESR、WBC 和 RBC）和生化参数（SOD、GSH、GR）以及血清标志物（CRP、RF）的影响。
AAEE 在疾病的发展和发展阶段均对多发性关节炎试验具有显著的抗关节炎活性。这与促炎介质（PGE2、NO、IL-1β 和 LTB4）、Th1-Th17 细胞因子（IL-2、TNF-α、IFN-γ、IL-12、IL-17、IL-6）和 Th2 细胞因子（IL-4 和 IL-10）的剂量依赖性抑制有关。AAEE 还观察到可保护大鼠免受原发性和继发性关节炎病变、体重变化和血液学紊乱的影响。此外，AAEE 对生化参数和血清标志物的抑制作用进一步证实，它降低了慢性炎症反应的迹象。
因此，本研究表明 AAEE 是一种有效的抗关节炎药物。对炎症介质和辅助性 T 细胞因子的多方面攻击以及 AAEE 的强大效力可能与抑制关节炎中的慢性炎症反应有关。
