State Key Laboratory of Agricultural Microbiology and College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, P. R. China.
Department of Biotechnology, Quaid-i-Azam University, Islamabad, 45320, Pakistan.
Sci Rep. 2018 Jul 23;8(1):11028. doi: 10.1038/s41598-018-29231-x.
Atropa acuminata Royle Ex Lindl (Atropa acuminata) under tremendous threat of extinction in its natural habitat. However, the antimicrobial, antileishmanial and anticancer effects of the plant's extracts have not been reported yet. In the current study, an attempt has been made to evaluate the pharmacological potential of this plant's extracts against microbes, Leishmania and cancer. The roots, stems and leaves of Atropa acuminata were ground; then, seven different solvents were used alone and in different ratios to prepare crude extracts, which were screened for pharmacological effects. The aqueous, methanolic and ethanolic extracts of all parts carried a broad spectrum of anti-bacterial activities, while no significant activity was observed with combined solvents. Three types of cytotoxicity assays were performed, i.e., haemolytic, brine shrimp and protein kinase assays. The aqueous extract of all the parts showed significant haemolytic activity while n-hexane extracts of roots showed significant activity against brine shrimp. The acetone extracts strongly inhibited protein kinase while the methanolic extracts exhibited significant cytotoxic activity of roots and stem. The anti-leishmanial assays revealed that the methanolic extract of leaves and roots showed significant activity. These findings suggest that this plant could be a potential source of natural product based drugs.
颠茄（Atropa acuminata）在其自然栖息地面临着灭绝的巨大威胁。然而，该植物提取物的抗菌、抗利什曼原虫和抗癌作用尚未得到报道。在本研究中，尝试评估该植物提取物对微生物、利什曼原虫和癌症的药理潜力。将颠茄的根、茎和叶研磨；然后，使用七种不同的溶剂单独和不同比例来制备粗提取物，以筛选其药理作用。所有部位的水提物、甲醇提物和乙醇提物均具有广谱的抗菌活性，而混合溶剂则没有观察到显著的活性。进行了三种类型的细胞毒性测定，即溶血、卤虫和蛋白激酶测定。所有部位的水提物均显示出显著的溶血活性，而根的正己烷提取物对卤虫表现出显著的活性。丙酮提取物强烈抑制蛋白激酶，而甲醇提取物则显示出根和茎的显著细胞毒性活性。抗利什曼原虫试验表明，叶和根的甲醇提取物具有显著的活性。这些发现表明，该植物可能是天然产物药物的潜在来源。
