Dzoyem J P, Eloff J N
Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, Pretoria, South Africa.
Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, Pretoria, South Africa.
J Ethnopharmacol. 2015 Feb 3;160:194-201. doi: 10.1016/j.jep.2014.11.034. Epub 2014 Dec 2.
Oxidative stress and inflammatory conditions are among the pathological features associated with the central nervous system in Alzheimer׳s disease. Traditionally, medicinal plants have been used to alleviate inflammation, pains and also other symptoms possibly associated with Alzheimer׳s disease. Therefore, the present study was designed to determine the in vitro anti-inflammatory, antioxidant and anticholinesterase activity of twelve South African medicinal plants traditionally used to alleviate pain and inflammation.
Nitric oxide (NO) production in LPS-activated RAW 264.7 macrophages and 15-lipoxygenase (LOX) inhibitory assay were used to evaluate the anti-inflammatory activity. Acetylcholinesterase inhibition was assessed by using a modification of the Ellman׳s method. Antioxidant activity, total phenolic and total flavonoids contents were determined using standard in vitro methods.
The extract of Burkea africana had the highest anti-15-lipoxygenase activity with 85.92% inhibition at 100µg/mL. All the extracts tested inhibited nitric oxide (NO) production in a dose dependant manner in LPS-stimulated RAW 264.7 macrophages. However, extracts from Leucaena leucocephala, Lippia javanica inhibited the production of NO by 97% at a concentration of 25µg/mL. In addition, both Leucaena leucocephala and Englerophytum magaliesmontanum had strong activity against acetylcholinesterase with IC50 values of 118µg/mL and 160µg/mL respectively. Hight levels of phenolics and flavonoids were found in Leucaena leucocephala, Lippia javanica and Burkea africana. The correlation with antioxidant activities was not strong indicating that other metabolites may also be involved in antioxidant activity.
The results obtained in this study validate the use of leaf extracts of these plants in South African traditional medicine against inflammation. Extracts of these plants species might be of value in the management of various diseases emerging from oxidative stress and related degenerative disorders.
氧化应激和炎症状态是阿尔茨海默病中枢神经系统相关的病理特征之一。传统上，药用植物被用于减轻炎症、疼痛以及其他可能与阿尔茨海默病相关的症状。因此，本研究旨在确定十二种南非传统上用于减轻疼痛和炎症的药用植物的体外抗炎、抗氧化和抗胆碱酯酶活性。
采用脂多糖激活的RAW 264.7巨噬细胞中一氧化氮（NO）生成及15-脂氧合酶（LOX）抑制试验来评估抗炎活性。通过改良的Ellman法评估乙酰胆碱酯酶抑制作用。采用标准体外方法测定抗氧化活性、总酚含量和总黄酮含量。
非洲伯克苏木提取物具有最高的抗15-脂氧合酶活性，在100μg/mL时抑制率为85.92%。所有测试提取物在脂多糖刺激的RAW 264.7巨噬细胞中均以剂量依赖性方式抑制一氧化氮（NO）生成。然而，银合欢、爪哇过江藤提取物在浓度为25μg/mL时对NO生成的抑制率达97%。此外，银合欢和马加利山裂果木对乙酰胆碱酯酶均有较强活性，IC50值分别为118μg/mL和160μg/mL。银合欢、爪哇过江藤和非洲伯克苏木中发现了高水平的酚类和黄酮类物质。与抗氧化活性的相关性不强，表明其他代谢物可能也参与了抗氧化活性。
本研究结果验证了这些植物叶提取物在南非传统医学中用于抗炎的用途。这些植物物种的提取物可能对管理由氧化应激和相关退行性疾病引发的各种疾病具有价值。
Zotero 插件