南非用于治疗与疼痛相关疾病的药用植物的抗炎、抗胆碱酯酶、抗氧化和植物化学特性。
Anti-inflammatory, anticholinesterase, antioxidant and phytochemical properties of medicinal plants used for pain-related ailments in South Africa.
机构信息
Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa.
出版信息
J Ethnopharmacol. 2010 Feb 3;127(2):235-41. doi: 10.1016/j.jep.2009.11.015. Epub 2009 Nov 20.
ETHNOPHARMACOLOGICAL RELEVANCE
Extracts of seven South African medicinal plants used traditionally for the treatment of pain-related ailments were evaluated.
AIMS OF THE STUDY
The study was aimed at evaluating medicinal and therapeutic potentials of the investigated traditional medicinal plants. Plant extracts were evaluated for anti-inflammatory activity and other pharmacological properties such as anticholinesterase and antioxidant activities. Phytochemical analysis of total phenolic contents, condensed tannins, gallotannins and flavonoids in the aqueous methanol extracts of the medicinal plants were also carried out.
MATERIALS AND METHODS
The evaluation of anti-inflammatory activity of 50% methanol (50% MeOH), petroleum ether (PE), dichloromethane (DCM) and ethanol (EtOH) plant extracts was done against cyclooxygenase-1 and -2 (COX-1 and COX-2) enzymes. 50% MeOH, PE, DCM and EtOH extracts were tested for acetylcholinesterase (AChE) inhibition, while 50% MeOH extracts were tested for 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activity and ferric-reducing power in the antioxidant assays. Total phenolic compounds, condensed tannins, gallotannins and flavonoids were quantitatively determined using spectrophotometric methods.
RESULTS
At the screening assay concentration (0.25 microg/microl), 13 extracts showed good COX-1 inhibitory activity (>50%), while good activity was observed in 15 extracts against COX-2 enzyme. All the extracts of Crinum moorei (bulbs) showed good inhibition against both COX-1 and COX-2 enzymes. Though not significantly different (P=0.05), the highest COX-1 percentage inhibition (100%) was shown by Aloe ferox leaf PE and Colocasia antiquorum tuber DCM extracts, while Colocasia antiquorum tuber PE extract exhibited the highest (92.7%) percentage inhibition against COX-2. Crinum moorei bulb DCM extract showed the lowest EC(50) value (2.9 microg/ml) in the AChE assay. In addition, good to moderate bioactivities were observed in some extracts of Aloe ferox (leaves), Crinum moorei (bulbs) and Pycnostachys reticulata (leaves) in all the assays. The presence and/or amounts of phenolic compounds varied with plant species.
CONCLUSION
The results obtained in this study validate the use of the investigated medicinal plants in South African traditional medicine for pain-related ailments.
民族药理学相关性
评估了七种南非传统药用植物的提取物，这些提取物用于治疗与疼痛相关的疾病。
研究目的
本研究旨在评估被调查的传统药用植物的药用和治疗潜力。评估植物提取物的抗炎活性以及其他药理学特性，如乙酰胆碱酯酶抑制和抗氧化活性。还对药用植物的水甲醇提取物中的总酚含量、缩合单宁、鞣花单宁和类黄酮进行了植物化学分析。
材料和方法
使用环加氧酶-1 和 -2（COX-1 和 COX-2）酶评估 50%甲醇（50%MeOH）、石油醚（PE）、二氯甲烷（DCM）和乙醇（EtOH）植物提取物的抗炎活性。测试 50%MeOH、PE、DCM 和 EtOH 提取物对乙酰胆碱酯酶（AChE）的抑制作用，而 50%MeOH 提取物则在抗氧化测定中测试 2,2-二苯基-1-苦基肼（DPPH）自由基清除活性和铁还原能力。使用分光光度法定量测定总酚类化合物、缩合单宁、鞣花单宁和类黄酮。
结果
在筛选试验浓度（0.25μg/μL）下，有 13 种提取物对 COX-1 具有良好的抑制活性（>50%），而有 15 种提取物对 COX-2 酶具有良好的活性。所有 Crinum moorei（鳞茎）的提取物对 COX-1 和 COX-2 酶均显示出良好的抑制作用。虽然没有显著差异（P=0.05），但 Aloe ferox 叶 PE 和 Colocasia antiquorum 块茎 DCM 提取物对 COX-1 的抑制率最高（100%），而 Colocasia antiquorum 块茎 PE 提取物对 COX-2 的抑制率最高（92.7%）。Crinum moorei 鳞茎 DCM 提取物在 AChE 测定中表现出最低的 EC（50）值（2.9μg/ml）。此外，在所有测定中，Aloe ferox（叶）、Crinum moorei（鳞茎）和 Pycnostachys reticulata（叶）的一些提取物表现出良好至中等的生物活性。酚类化合物的存在和/或含量随植物种类而变化。
结论
本研究的结果验证了南非传统医学中使用所研究的药用植物治疗与疼痛相关的疾病的用途。
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