Kang Wenjing, Pei Xing, Bange Adam, Haynes Erin N, Heineman William R, Papautsky Ian
BioMicroSystems Lab, Department of Electrical Engineering and Computing Systems, University of Cincinnati , Cincinnati, Ohio 45221-0030, United States.
Anal Chem. 2014 Dec 16;86(24):12070-7. doi: 10.1021/ac502882s. Epub 2014 Dec 5.
In this work, we report on the development of a palladium-based, microfabricated point-of-care electrochemical sensor for the determination of manganese using square wave cathodic stripping voltammetry. Heavy metals require careful monitoring, yet current methods are too complex for a point-of-care system. Voltammetry offers an attractive approach to metal detection on the microscale, but traditional carbon, gold, or platinum electrodes are difficult or expensive to microfabricate, preventing widespread use. Our sensor uses palladium working and auxiliary electrodes and integrates them with a copper-based reference electrode for simple fabrication and compatibility with microfabrication and printed circuit board processing, while maintaining competitive performance in electrochemical detection. Copper electrodes were prepared on glass substrate using a combination of microfabrication procedures followed by electrodeposition of palladium. The disposable sensor system was formed by bonding a poly(dimethylsiloxane) (PDMS) well to the glass substrate. Cathodic stripping voltammetry of manganese using our new disposable palladium-based sensors exhibited 334 nM (18.3 ppb) limit of detection in borate buffer. The sensor was used to demonstrate manganese determination in natural water samples from a pond in Burnet Woods, located in Cincinnati, OH, and the Ohio River.
在本研究中，我们报告了一种基于钯的微制造即时检测电化学传感器的开发，该传感器采用方波阴极溶出伏安法测定锰。重金属需要仔细监测，但目前的方法对于即时检测系统来说过于复杂。伏安法为微尺度上的金属检测提供了一种有吸引力的方法，但传统的碳、金或铂电极难以微制造或成本过高，阻碍了其广泛应用。我们的传感器使用钯工作电极和辅助电极，并将它们与铜基参比电极集成在一起，便于制造且与微制造和印刷电路板工艺兼容，同时在电化学检测中保持具有竞争力的性能。通过微制造程序的组合，随后电沉积钯，在玻璃基板上制备铜电极。一次性传感器系统通过将聚二甲基硅氧烷（PDMS）池与玻璃基板键合而成。使用我们新型一次性钯基传感器对锰进行阴极溶出伏安法检测，在硼酸盐缓冲液中的检测限为334 nM（18.3 ppb）。该传感器用于测定位于俄亥俄州辛辛那提市伯内特伍兹的一个池塘以及俄亥俄河采集的天然水样中的锰。
