Wu Zhizhen, Heineman William R, Haynes Erin N, Papautsky Ian
Department of Biomedical Engineering, University of Illinois Chicago, Illinois 60607, USA.
Department of Chemistry, University of Cincinnati, Cincinnati, Ohio 45221, USA.
J Electrochem Soc. 2022 May;169(5). doi: 10.1149/1945-7111/ac6a19. Epub 2022 May 11.
In this work, we demonstrate accurate and precise measurement of manganese (Mn) concentration in human whole blood with indium tin oxide (ITO) electrode using square wave stripping voltammetry. While an essential trace metal for human health, elevated levels of Mn due to environmental or occupational exposure have been associated with severe neuromotor dysfunction characterized by parkinsonism and cognitive dysfunction making the monitoring of Mn in whole blood necessary. Pediatric populations are particularly susceptible to Mn given their developing brain and potential long-term impacts on neurodevelopment. The current gold standard for whole blood Mn measurements is by ICP-MS, which is costly and time consuming. The electrochemical detection with ITO working electrode in this work showed a limit of detection of 0.5 μg l and a linear range of 5 to 500 μg l, which encompasses the physiological Mn levels in human whole blood (5-18 μg l). Our results of Mn measurement in whole blood show an average precision of 96.5% and an average accuracy of 90.3% compared to ICP-MS for both the normal range (5-18 μg l) and the elevated levels (>36 μg l) that require medical intervention. These results demonstrate the feasibility of Mn measurements in human blood with electrochemical sensors.
在本研究中，我们使用方波溶出伏安法，通过氧化铟锡（ITO）电极展示了对人全血中锰（Mn）浓度的准确和精确测量。锰是人体健康必需的痕量金属，然而，由于环境或职业暴露导致的锰水平升高与以帕金森症和认知功能障碍为特征的严重神经运动功能障碍有关，因此监测全血中的锰十分必要。鉴于儿童大脑仍在发育，且锰对神经发育可能产生长期影响，儿童群体尤其易受锰的影响。目前全血锰测量的金标准是电感耦合等离子体质谱法（ICP-MS），该方法成本高且耗时。本研究中使用ITO工作电极进行的电化学检测显示，检测限为0.5μg/L，线性范围为5至500μg/L，涵盖了人全血中的生理锰水平（5 - 18μg/L）。我们对全血中锰的测量结果表明，与ICP-MS相比，对于正常范围（5 - 18μg/L）和需要医疗干预的升高水平（>36μg/L），平均精密度为96.5%，平均准确度为90.3%。这些结果证明了使用电化学传感器测量人体血液中锰的可行性。
