Haanstra Jurgen R, Bakker Barbara M, Michels Paul A M
Department of Pediatrics and Systems Biology Centre for Energy Metabolism and Ageing, Centre for Liver, Digestive and Metabolic Diseases, University of Groningen, University Medical Center Groningen, The Netherlands; Department of Molecular Cell Physiology, Amsterdam Institute for Molecules, Medicines and Systems (AIMMS), Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, The Netherlands.
Department of Pediatrics and Systems Biology Centre for Energy Metabolism and Ageing, Centre for Liver, Digestive and Metabolic Diseases, University of Groningen, University Medical Center Groningen, The Netherlands; Department of Molecular Cell Physiology, Amsterdam Institute for Molecules, Medicines and Systems (AIMMS), Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, The Netherlands.
Mol Biochem Parasitol. 2014 Nov;198(1):18-28. doi: 10.1016/j.molbiopara.2014.11.004. Epub 2014 Dec 2.
Trypanosomatids sequester large parts of glucose metabolism inside specialised peroxisomes, called glycosomes. Many studies have shown that correct glycosomal compartmentalization of glycolytic enzymes is essential for bloodstream-form Trypanosoma brucei. The recent finding of pore-forming activities in glycosomal membrane preparations and extensions of the trypanosome glycolysis computer model with size-selective pores sparked again an old debate on the extent of (im)permeability of the glycosomal membrane and whether glycosomally located glycolytic enzymes could and should also be present with some activity in the cytosol. This review presents a critical discussion of the experimental and theoretical evidence for and against the different hypotheses.
锥虫将葡萄糖代谢的大部分过程隔离在称为糖体的特殊过氧化物酶体中。许多研究表明，糖酵解酶在糖体中的正确区室化对于布氏锥虫的血流形式至关重要。最近在糖体膜制剂中发现的成孔活性以及锥虫糖酵解计算机模型中具有尺寸选择性孔的扩展，再次引发了关于糖体膜（不）通透性程度以及糖体定位的糖酵解酶是否能够且应该在细胞质中也具有一定活性的古老争论。本综述对支持和反对不同假设的实验和理论证据进行了批判性讨论。
