Wang Shaohua, Li Hanjun, Wang Jingjie, Wang Dan, Yao Anlong, Li Qiurong
Department of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, 305 Zhongshan East Road, Nanjing, Jiangsu 210002, China.
Dis Markers. 2014;2014:401986. doi: 10.1155/2014/401986. Epub 2014 Nov 12.
The purpose of this study was to determine the expression of miR-127 and analyze its prognostic and biological significance in breast cancer (BC). A quantitative reverse transcription PCR assay was performed to detect the expression of miR-127 in 15 pairs of BC and corresponding noncancerous tissues. The expression of miR-127 was detected in another 110 BC tissues and its correlations with clinicopathological factors of patients were examined. Univariate and multivariate analyses were performed to analyze the prognostic significance of miR-127 expression. The effects of miR-127 expression on malignant phenotypes of BC cells and its possible molecular mechanisms were further determined. miR-127 was significantly downregulated in BC tissues, and low miR-127 expression was significantly correlated with lymph node metastasis and advanced clinical stage. Patients with low miR-127 showed poorer overall survival than those with high miR-127. Multivariate analyses indicated that status of miR-127 was an independent prognostic factor for patients. Functional analyses showed that upregulation of miR-127 significantly inhibited growth, enhanced apoptosis, and reduced migration and invasion in BC cells by targeting the protooncogene BCL-6. Therefore, miR-127 may be a potential biomarker for predicting the survival of BC patients and might be a molecular target for treatment of human BCs.
本研究的目的是确定miR-127的表达，并分析其在乳腺癌（BC）中的预后和生物学意义。采用定量逆转录PCR检测15对乳腺癌组织及相应癌旁组织中miR-127的表达。在另外110例乳腺癌组织中检测miR-127的表达，并分析其与患者临床病理因素的相关性。进行单因素和多因素分析以分析miR-127表达的预后意义。进一步确定miR-127表达对乳腺癌细胞恶性表型的影响及其可能的分子机制。miR-127在乳腺癌组织中显著下调，低miR-127表达与淋巴结转移和临床分期进展显著相关。miR-127低表达的患者总生存期较miR-127高表达的患者差。多因素分析表明，miR-127状态是患者的独立预后因素。功能分析表明，上调miR-127可通过靶向原癌基因BCL-6显著抑制乳腺癌细胞生长、增强凋亡并减少迁移和侵袭。因此，miR-127可能是预测乳腺癌患者生存的潜在生物标志物，也可能是治疗人类乳腺癌的分子靶点。
