Rahmani Samani Mahsa, Zarif-Yeganeh Marjan, Mehrabi Atefeh, Emami Razavi Amir Nader, Sheikholeslami Sara, Hedayati Mehdi
School of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences and Metabolism, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Public Health. 2021 Feb;50(2):391-396. doi: 10.18502/ijph.v50i2.5358.
Medullary thyroid cancer (MTC) accounts for 5%-10% of all thyroid cancers, but causes 13% of all thyroid cancer related deaths. MicroRNAs (miRs) have key functions in the development and progression of MTC. Altered expression of some miRs has been reported in many human cancers, including Thyroid cancer. Therefore, we aimed to analyze the expression of miR-154, miR-183 and miR-127 in MTC tumor tissues.
In this case-control study, 15 MTC Formalin-fixed, paraffin-embedded (FFPE) tissue samples and 15 adjacent normal thyroid FFPE tissues, as a control group, were collected from Taleghani, and Loghman Hakim Hospitals, Tehran, Iran since 2005 till 2015. After RNA extraction and cDNA synthesis, the expression of miR-127, miR-154 and miR-183 was measured by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR).
Our data showed a significant increase in the expression of miR-127 in MTC samples in comparison with the control group (<0.05). Although miR-154 and miR-183 expression levels had increase expression in MTC tumors, this change was not statistically significant.
The miR-127 could be considered as a prognostic, diagnostic and therapeutic marker for the management of MTC, and it is proposed for further investigation to fully establish the role of this miRNA in MTC.
甲状腺髓样癌（MTC）占所有甲状腺癌的5%-10%，但却导致了13%的甲状腺癌相关死亡。微小RNA（miRs）在MTC的发生和发展中起关键作用。在包括甲状腺癌在内的许多人类癌症中，已报道某些miRs的表达发生改变。因此，我们旨在分析miR-154、miR-183和miR-127在MTC肿瘤组织中的表达。
在这项病例对照研究中，自2005年至2015年，从伊朗德黑兰的塔莱加尼医院和洛格曼·哈基姆医院收集了15份MTC福尔马林固定、石蜡包埋（FFPE）组织样本以及15份相邻的正常甲状腺FFPE组织作为对照组。经过RNA提取和cDNA合成后，通过定量实时聚合酶链反应（qRT-PCR）测量miR-127、miR-154和miR-183的表达。
我们的数据显示，与对照组相比，MTC样本中miR-127的表达显著增加（<0.05）。虽然miR-154和miR-183在MTC肿瘤中的表达水平有所升高，但这种变化在统计学上并不显著。
miR-127可被视为MTC管理中的一种预后、诊断和治疗标志物，建议进一步研究以充分确定这种微小RNA在MTC中的作用。
