Alzate Oscar, Osorio Cristina, DeKroon Robert M, Corcimaru Ana, Gunawardena Harsha P
Systems Proteomics Center, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA ; School of Medicine, Universidad Pontificia Bolivariana, Medellin, Colombia ; Current address: 108 Reynolds Medical Building, College of Medicine, Texas A&M Health Science Center, College Station, TX 77843-1114, USA.
Systems Proteomics Center, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Alzheimers Res Ther. 2014 Jul 14;6(4):43. doi: 10.1186/alzrt273. eCollection 2014.
Alzheimer's disease (AD) is the major cause of dementia among the elderly. Finding blood-based biomarkers for disease diagnosis and prognosis is urgently needed.
We studied protein distributions in brain tissues, cerebrospinal fluid (CSF), and blood of AD patients by using proteomics and a new proteomic method that we call "2D multiplexed Western blot" (2D mxWd). This method allows us to determine in multiple samples the electrophoretic patterns of protein isoforms with different isoelectric points.
Apolipoprotein E (ApoE) displays a unique distribution of electrophoretic isoforms in the presence of AD and also a unique pattern specific to the APOE genotype.
The isoelectric distribution of differentially charged ApoE isoforms was used to determine the presence of AD in a small group of samples. Further studies are needed to validate their use as predictors of disease onset and progression, and as biomarkers for determining the efficacy of therapeutic treatments.
阿尔茨海默病（AD）是老年人痴呆的主要病因。迫切需要找到基于血液的生物标志物用于疾病诊断和预后评估。
我们通过蛋白质组学以及一种我们称为“二维多重蛋白质印迹法”（2D mxWd）的新蛋白质组学方法，研究了AD患者脑组织、脑脊液（CSF）和血液中的蛋白质分布。该方法使我们能够在多个样本中确定具有不同等电点的蛋白质亚型的电泳图谱。
载脂蛋白E（ApoE）在AD存在时呈现出独特的电泳亚型分布，并且具有特定于APOE基因型的独特模式。
利用带不同电荷的ApoE亚型的等电分布来确定一小部分样本中AD的存在。需要进一步研究来验证它们作为疾病发作和进展预测指标以及确定治疗效果生物标志物的用途。
