Mabruk M J, Glasglow G M, Flack A M, Folan J C, Bannigan J G, Smyth J M, O'Sullivan M A, Sheahan B J, Atkins G J
Department of Microbiology, Moyne Institute, Trinity College, Dublin, Ireland.
J Virol. 1989 Sep;63(9):4027-33. doi: 10.1128/JVI.63.9.4027-4033.1989.
The A7 strain of Semliki Forest virus induces rapid fetal death in pregnant mice, whereas the ts22 mutant derived from it is teratogenic for a proportion of fetuses. Both A7 and ts22 induce viremia and infect the central nervous systems and fetuses of pregnant mice. Using immunogold-silver staining, a cDNA probe for a Semliki Forest virus nonstructural sequence, and a riboprobe derived from the same sequence, we showed that the skin and musculoskeletal systems of fetuses from mothers infected with ts22 were often heavily infected but the central nervous systems were not labeled before day 17 of pregnancy. Damage to the neural tube, including open-neural-tube defects, was detected in fetuses following infection of the mother at days 8 and 10 of pregnancy with both A7 and ts22. For ts22, neural tube damage induced by fetal infection before day 17 of pregnancy appeared to be indirect and caused by virus infection of mesenchymal cells surrounding the developing neural tube.
塞姆利基森林病毒的A7毒株可导致怀孕小鼠的胎儿迅速死亡，而由它衍生出的ts22突变株对一部分胎儿具有致畸性。A7和ts22均可引发病毒血症，并感染怀孕小鼠的中枢神经系统和胎儿。我们运用免疫金银染色法、一种针对塞姆利基森林病毒非结构序列的cDNA探针以及源自同一序列的核糖探针，发现感染ts22的母亲所生胎儿的皮肤和肌肉骨骼系统常常受到严重感染，但在怀孕第17天之前，中枢神经系统并未被标记。在怀孕第8天和第10天，用A7和ts22感染母亲后，在胎儿中检测到神经管损伤，包括开放性神经管缺陷。对于ts22而言，怀孕第17天之前胎儿感染所引发的神经管损伤似乎是间接的，是由发育中的神经管周围间充质细胞的病毒感染所致。
