Gotoh H, Shioda T, Sakai Y, Mizumoto K, Shibuta H
Department of Viral Infection, The University of Tokyo, Japan.
Virology. 1989 Aug;171(2):434-43. doi: 10.1016/0042-6822(89)90612-0.
The Sendai virus ribonucleoprotein (RNP) showed only very low plaque-forming titers upon transfection and the virus yields after one-step growth were quite limited. We tried to enhance the Sendai virus yield by supplying the viral L and P/C gene products through vaccinia vectors. A combination of the recombinant vaccinia viruses carrying the L gene (Vac-HL) and the P/C gene (Vac-HPC), both of which were driven by the promoter of the vaccinia virus 7.5K protein gene, enhanced the yield only a little whereas another combination of Vac-HLd7.5, the L gene insert of which was driven by the promoter of the vaccinia virus thymidine kinase gene in place of the 7.5K promoter, and Vac-HPC greatly enhanced the Sendai virus yield. This seemed to correlate with the fact that the Vac-HL interfered with Sendai virus growth markedly while the Vac-HLd7.5 did not. These results strongly suggest that the L and P/C gene products act in cooperation as the RNA polymerase, and overproduction of the L protein is inhibitory for Sendai virus growth. This system seems to be of value as a tool for analyzing the functions of L and P/C genes of Sendai virus.
仙台病毒核糖核蛋白（RNP）转染后仅表现出非常低的蚀斑形成滴度，一步生长后的病毒产量也相当有限。我们试图通过痘苗载体提供病毒L和P/C基因产物来提高仙台病毒产量。携带L基因的重组痘苗病毒（Vac-HL）和携带P/C基因的重组痘苗病毒（Vac-HPC）的组合，两者均由痘苗病毒7.5K蛋白基因的启动子驱动，产量仅略有提高，而另一种组合Vac-HLd7.5（其L基因插入片段由痘苗病毒胸苷激酶基因的启动子代替7.5K启动子驱动）和Vac-HPC则极大地提高了仙台病毒产量。这似乎与Vac-HL显著干扰仙台病毒生长而Vac-HLd7.5不干扰这一事实相关。这些结果有力地表明，L和P/C基因产物作为RNA聚合酶协同发挥作用，L蛋白的过量产生对仙台病毒生长具有抑制作用。该系统作为分析仙台病毒L和P/C基因功能的工具似乎具有价值。
