Wesley I V, Bryner J H
National Animal Disease Center, US Department of Agriculture, Ames, IA 50010.
Am J Vet Res. 1989 Jun;50(6):807-13.
Thirty-two isolates of Campylobacter fetus subsp venerealis were obtained from 1 bull and 4 heifers with experimentally induced infection. When whole-cell antigens of isolates were cross titrated with antisera to the infecting strain, isolates from 3 heifers had limited antigenic variation, whereas whole-cell antigens of isolates from 2 cattle (the bull and a heifer) differed serologically from those of the infecting strain. Changes were detected specifically in 6 heat-labile antigens. Of the 6 heat-labile factors evaluated, all were initially present on the infecting parent strain, but not on early isolates obtained from 4 of the 5 cattle. Restriction enzyme analysis revealed minor variation in the DNA fingerprints of isolates obtained from individual cattle, thus implying stability of the Campylobacter genome once persistent infection is established. Isolates with identical restriction enzyme patterns expressed different heat-labile antigens. Correlation could not be found between the DNA electrophoretic pattern and the expression of heat-labile antigens.
从1头公牛和4头经实验诱导感染的小母牛中获得了32株胎儿弯曲杆菌性病亚种菌株。当用针对感染菌株的抗血清对菌株的全细胞抗原进行交叉滴定分析时，发现来自3头小母牛的菌株抗原变异有限，而来自2头牛（1头公牛和1头小母牛）的菌株全细胞抗原在血清学上与感染菌株不同。在6种热不稳定抗原中检测到了变化。在评估的6种热不稳定因子中，所有因子最初都存在于感染的亲本菌株上，但在从5头牛中的4头牛获得的早期菌株中不存在。限制性内切酶分析显示，从个体牛获得的菌株的DNA指纹图谱存在微小差异，这意味着一旦建立持续感染，弯曲杆菌基因组具有稳定性。具有相同限制性内切酶图谱的菌株表达不同的热不稳定抗原。在DNA电泳图谱与热不稳定抗原的表达之间未发现相关性。
