Wesley I V, Baetz A L, Larson D J
Enteric Diseases and Food Safety Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 50010, USA.
Infect Immun. 1996 Jun;64(6):2295-9. doi: 10.1128/iai.64.6.2295-2299.1996.
Neonatal piglets have been used as models to study human campylobacteriosis and helicobacteriosis. The purpose of this study was to determine the relative pathogenicities, on the basis of the duration of fecal shedding and colonization of tissues, of three Arcobacter species in 1-day-old cesarean-derived colostrum-deprived piglets. Two experiments were conducted. In experiment 1, two piglets each were infected per os with either Arcobacter butzleri ATCC 49616, Arcobacter cryaerophilus 1B ATCC 43159, Arcobacter skirrowii CCUG 10374, or the three field strains of A. butzleri (approximately 5 X 10(9) CFU per piglet). Rectal swab samples were taken prior to infection and daily thereafter for up to 7 days. Arcobacter spp. were detected at least once in rectal swab samples of all but one of the experimentally infected piglets but not in the control. At necropsy, A. butzleri was recovered from the lung, kidney, ileum, or brain tissues of the four infected piglets which had received either the field strain or the ATCC type strain of A. butzleri. A. cryaerophilus 1B was detected in rectal swab samples for up to 7 days postinfection but was not cultured from tissues at necropsy. Arcobacters were detected in the rectal swab sample of the A. skirrowii-infected piglet only on day 3 postinfection; no isolates were obtained from tissues at necropsy. No gross pathological lesions were consistently noted in the experimentally infected piglets. In experiment 2, two piglets each were infected per os with A. butzleri ATCC 49616, A. cryaerophilus 1A ATCC 43158, A. skirrowii CCUG 10374, or the single A. butzleri field strain Yard J/c (approximately 5 X 10(9) CFU per piglet). Arcobacter spp. were cultured from rectal swab samples of all but one of the experimentally infected piglets at least once. At necropsy Arcobacter spp. were cultured from the liver, kidney, ileum, or brain tissues of two of the four A. butzleri-infected piglets. However, no severe gross pathology was noted. These data suggest that Arcobacter spp., especially A. butzleri, can colonize neonatal pigs.
新生仔猪已被用作研究人类弯曲菌病和螺杆菌病的模型。本研究的目的是根据粪便排菌持续时间和组织定植情况，确定三种弓形杆菌对1日龄剖宫产初乳剥夺仔猪的相对致病性。进行了两项实验。在实验1中，每头仔猪经口感染以下菌株：布氏弓形杆菌ATCC 49616、嗜低温弓形杆菌1B ATCC 43159、斯氏弓形杆菌CCUG 10374，或三种布氏弓形杆菌野外菌株（每头仔猪约5×10⁹CFU）。在感染前采集直肠拭子样本，此后每天采集，持续7天。除一头实验感染仔猪外，所有实验感染仔猪的直肠拭子样本中至少检测到一次弓形杆菌属，但对照组未检测到。剖检时，从接受布氏弓形杆菌野外菌株或ATCC标准菌株的四只感染仔猪的肺、肾、回肠或脑组织中分离出布氏弓形杆菌。嗜低温弓形杆菌1B在感染后7天内的直肠拭子样本中均可检测到，但剖检时未从组织中培养出该菌。斯氏弓形杆菌感染的仔猪仅在感染后第3天的直肠拭子样本中检测到弓形杆菌；剖检时未从组织中分离到菌株。实验感染仔猪未发现一致的明显病理病变。在实验2中，每头仔猪经口感染以下菌株：布氏弓形杆菌ATCC 49616、嗜低温弓形杆菌1A ATCC 43158、斯氏弓形杆菌CCUG 10374，或单一的布氏弓形杆菌野外菌株Yard J/c（每头仔猪约5×10⁹CFU）。除一头实验感染仔猪外，所有实验感染仔猪的直肠拭子样本中至少一次培养出弓形杆菌属。剖检时，四只布氏弓形杆菌感染仔猪中的两只的肝、肾、回肠或脑组织中培养出弓形杆菌属。然而，未发现严重的明显病理变化。这些数据表明，弓形杆菌属，尤其是布氏弓形杆菌，可在新生仔猪中定植。
