Johnson Ryan C, Ellis Michael W, Lanier Jeffrey B, Schlett Carey D, Cui Tianyuan, Merrell D Scott
Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA.
Department of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA.
Infect Immun. 2015 Feb;83(2):802-11. doi: 10.1128/IAI.02664-14. Epub 2014 Dec 8.
The incidence of skin and soft tissue infections (SSTIs) has increased dramatically over the past decade, resulting in significant morbidity in millions of otherwise healthy individuals worldwide. Certain groups, like military personnel, are at increased risk for SSTI development. Although nasal colonization with Staphylococcus aureus is an important risk factor for the development of SSTIs, it is not clear why some colonized individuals develop disease while others do not. Recent studies have revealed the importance of microbial diversity in human health. Therefore, we hypothesized that the nasal microbiome may provide valuable insight into SSTI development. To examine this hypothesis, we obtained anterior-naris samples from military trainees with cutaneous abscesses and from asymptomatic (non-SSTI) participants. We also obtained samples from within abscess cavities. Specimens were analyzed by culture, and the microbial community within each sample was characterized using a 16S sequencing-based approach. We collected specimens from 46 non-SSTI participants and from 40 participants with abscesses. We observed a significantly higher abundance of Proteobacteria in the anterior nares in non-SSTI participants (P < 0.0001) than in participants with abscesses. Additionally, we noted a significant inverse correlation between Corynebacterium spp. and S. aureus (P = 0.0001). The sensitivity of standard microbiological culture for abscesses was 71.4%. These data expand our knowledge of the complexity of the nasal and abscess microbiomes and potentially pave the way for novel therapeutic and prophylactic countermeasures against SSTI.
在过去十年中，皮肤和软组织感染（SSTIs）的发病率急剧上升，导致全球数百万原本健康的个体出现严重发病情况。某些群体，如军事人员，发生SSTIs的风险增加。虽然金黄色葡萄球菌的鼻腔定植是SSTIs发生的一个重要风险因素，但尚不清楚为什么一些定植个体发病而另一些不发病。最近的研究揭示了微生物多样性对人类健康的重要性。因此，我们假设鼻腔微生物群可能为SSTIs的发生提供有价值的见解。为了验证这一假设，我们从患有皮肤脓肿的军事训练人员和无症状（非SSTI）参与者中获取了前鼻孔样本。我们还从脓肿腔内获取了样本。通过培养对标本进行分析，并使用基于16S测序的方法对每个样本中的微生物群落进行表征。我们从46名非SSTI参与者和40名患有脓肿的参与者中收集了标本。我们观察到，非SSTI参与者前鼻孔中变形菌门的丰度显著高于患有脓肿的参与者（P < 0.0001）。此外，我们注意到棒状杆菌属与金黄色葡萄球菌之间存在显著的负相关（P = 0.0001）。脓肿标准微生物培养的敏感性为71.4%。这些数据扩展了我们对鼻腔和脓肿微生物群复杂性的认识，并可能为针对SSTI的新型治疗和预防措施铺平道路。
