甲型肝炎减毒活疫苗的试制

[The trial preparation of attenuated live hepatitis A vaccine].

作者信息

Dong D X

出版信息

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1989 Feb;11(1):1-6.

Abstract

The experimental attenuated live hepatitis A vaccine has been prepared in KMB17 human fetal lung diploid cells with the HAV variant H2M20K5 (32 degrees C). No extraneous agent was detected in the vaccine, which had a titer of 10(6.5) TCID50/ml. Twelve monkeys vaccinated intravenously or subcutaneously were found to have no significant clinical hepatitis but with anti-HAV antibody in all cases. Thus the safety and immunogenicity of the vaccine were evidenced in monkeys and it is necessary to investigate the trial vaccination further in a few susceptible individuals.

摘要

实验性减毒活甲型肝炎疫苗是在KMB17人胚肺二倍体细胞中采用甲型肝炎病毒变异株H2M20K5（32℃）制备的。疫苗中未检测到外源因子，其滴度为10(6.5) TCID50/ml。给12只猴子静脉或皮下接种疫苗后，发现它们均未出现明显的临床肝炎症状，但所有猴子均产生了抗甲型肝炎病毒抗体。因此，该疫苗在猴子身上证明了安全性和免疫原性，有必要在少数易感个体中进一步开展试验性接种研究。