Huang X, Lian Y, Wang Q
Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Kunming.
Zhonghua Yu Fang Yi Xue Za Zhi. 1997 Sep;31(5):260-2.
Common marmosets with negative serum antibodies against hepatitis A virus (anti-HAV) and normal liver functions were used for the tests of passage of H2 vaccine strain of HAV to explore its residual virulence after attenuation and possibility of its reversion. Common marmosets were inoculated with attenuated live vaccine, and fecal suspension and/or proliferative liquid in tissue culture of the progeny of vaccine HAV excreted from their feces were passaged in common marmosets. Result of three serial passages in vivo indicated that there was no significant difference in serum anti-HAV titer, and no or low-level changes in activities of liver enzymes, no pathological changes in liver tissues observed, and detectable but minimal HAV was shed in stool of the all animals.
选用血清抗甲型肝炎病毒（抗-HAV）阴性且肝功能正常的普通狨猴进行甲型肝炎病毒H2疫苗株传代试验，以探索其减毒后的残余毒力及返祖可能性。给普通狨猴接种减毒活疫苗，并将从其粪便中排出的疫苗甲型肝炎病毒子代的粪便悬液和/或组织培养增殖液在普通狨猴体内传代。体内连续传代3次的结果表明，血清抗-HAV滴度无显著差异，肝酶活性无变化或仅有低水平变化，未观察到肝组织病理变化，所有动物粪便中均可检测到但含量极低的甲型肝炎病毒。
