Hryniewicka Marta, Starczewska Barbara
Institute of Chemistry, University of Białystok, ul. Hurtowa 1, 15-399 Białystok, Poland.
Institute of Chemistry, University of Białystok, ul. Hurtowa 1, 15-399 Białystok, Poland.
J Pharm Biomed Anal. 2015 Mar 15;106:129-35. doi: 10.1016/j.jpba.2014.11.030. Epub 2014 Nov 22.
This work illustrates the development of new procedures for the isolation and preconcentration of fluvastatin (FLU) from aqueous solutions. Micellar extraction (ME) combined with high performance liquid chromatography (HPLC-UV) has been successfully applied for this purpose. It was found that the analyte created micelle with anionic sodium dodecylsulfate (SDS) and/or with the binary mixture of surfactants nonionic triton X114 (TX114) and cationic tetra-n-butyloammonium bromide (TBAB). The optimal analytical conditions for the proposed extraction procedures (solution pH, concentration of surfactants, centrifugation time and electrolyte type) were ascertained. The calibration curves were recorded. The linearity ranges for FLU, isolated by SDS and the mixture of TX114/TBAB, were 0.21-28.79 μg mL(-1) and 0.21-16.45 μg mL(-1) with limit of detection (LOD) 0.19 μg mL(-1) and 0.14 μg mL(-1), respectively. The recoveries afforded by the proposed methods were high, approximately 97%. These preconcentration procedures were applied for the isolation of the statin from water and wastewater samples taken from the local rivers and wastewater treatment plants.
这项工作展示了从水溶液中分离和预富集氟伐他汀（FLU）的新方法的开发。胶束萃取（ME）与高效液相色谱法（HPLC - UV）相结合已成功用于此目的。研究发现，分析物与阴离子十二烷基硫酸钠（SDS）和/或与非离子表面活性剂曲拉通X114（TX114）和阳离子四丁基溴化铵（TBAB）的二元混合物形成胶束。确定了所提出的萃取方法的最佳分析条件（溶液pH值、表面活性剂浓度、离心时间和电解质类型）。记录了校准曲线。通过SDS和TX114/TBAB混合物分离的FLU的线性范围分别为0.21 - 28.79 μg mL⁻¹和0.21 - 16.45 μg mL⁻¹，检测限（LOD）分别为0.19 μg mL⁻¹和0.14 μg mL⁻¹。所提出方法的回收率很高，约为97%。这些预富集方法用于从当地河流和废水处理厂采集的水和废水样品中分离他汀类药物。
