Balentova Sona, Hajtmanova Eva, Adamkov Marian, Lehotsky Jan
Jessenius Faculty of Medicine in Martin, Institute of Histology and Embryology, Comenius University in Bratislava, Mala Hora 4, 036 01, Martin, Slovak Republic,
Neurochem Res. 2015 Mar;40(3):501-13. doi: 10.1007/s11064-014-1495-8. Epub 2014 Dec 9.
Ionizing radiation induces altered brain tissue homeostasis and can lead to morphological and functional deficits. In this study, adult male Wistar rats received whole-body exposure with fractionated doses of gamma rays (a total dose of 5 Gy) and were investigated 30 and 60 days later. Immunohistochemistry and confocal microscopy were used to determine proliferation rate of cells residing or derived from the forebrain anterior subventricular zone (SVZa) and microglia distributed along and/or adjacent to subventricular zone-olfactory bulb axis. Cell counting was performed in four anatomical parts along the well-defined pathway, known as the rostral migratory stream (RMS) represented by the SVZa, vertical arm, elbow and horizontal arm of the RMS. Different spatiotemporal distribution pattern of cell proliferation was seen up to 60 days after irradiation through the migratory pathway. A population of neuroblasts underwent less evident changes up to 60 days after treatment. Fractionated exposure led to decline or loss of resting as well as reactive forms of microglia until 60 days after irradiation. Results showed that altered expression of the SVZa derived cells and ultimative decrease of microglia may contribute to development of radiation-induced late effects.
电离辐射会导致脑组织内环境稳态改变，并可能引发形态和功能缺陷。在本研究中，成年雄性Wistar大鼠接受了分次剂量的γ射线全身照射（总剂量5 Gy），并在30天和60天后进行了研究。采用免疫组织化学和共聚焦显微镜技术，确定位于前脑前脑室下区（SVZa）或源自该区域的细胞以及沿脑室下区-嗅球轴分布和/或相邻的小胶质细胞的增殖率。在沿明确路径的四个解剖部位进行细胞计数，该路径称为由SVZa代表的吻侧迁移流（RMS）、RMS的垂直臂、肘部和水平臂。在照射后长达60天的时间里，通过迁移路径观察到细胞增殖的不同时空分布模式。一群神经母细胞在治疗后60天内变化不太明显。分次照射导致静息型和反应型小胶质细胞数量减少或丧失，直至照射后60天。结果表明，SVZa衍生细胞的表达改变和小胶质细胞的最终减少可能导致辐射诱发的晚期效应的发生。
