Department of Medical Biophysics, University of Western Ontario, London, Ontario, Canada.
Biol Proced Online. 2011 Oct 19;13(1):9. doi: 10.1186/1480-9222-13-9.
A cell-counting algorithm, developed in Matlab®, was created to efficiently count migrated fluorescently-stained cells on membranes from migration assays. At each concentration of cells used (10,000, and 100,000 cells), images were acquired at 2.5 ×, 5 ×, and 10 × objective magnifications. Automated cell counts strongly correlated to manual counts (r2 = 0.99, P < 0.0001 for a total of 47 images), with no difference in the measurements between methods under all conditions. We conclude that our automated method is accurate, more efficient, and void of variability and potential observer bias normally associated with manual counting.
我们开发了一种细胞计数算法，用于高效地计算迁移实验中膜上荧光染色细胞的数量。在使用的每个细胞浓度（10000 个和 100000 个细胞）下，我们在 2.5×、5×和 10×物镜放大倍数下获取图像。自动细胞计数与手动计数强烈相关（总共 47 张图像的 r2=0.99，P<0.0001），在所有条件下，两种方法的测量值没有差异。我们得出结论，我们的自动方法准确、高效，并且没有手动计数通常存在的变异性和潜在观察者偏差。
