Streptomyces xiaopingdaonensis sp. nov., a novel marine actinomycete isolated from the sediment of Xiaopingdao in Dalian, China.

A novel streptomycete, designated as strain DUT 180(T), was isolated from a marine sediment sample collected from a sea cucumber farm in Dalian, northeast China. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DUT 180(T) is phylogenetically affiliated to the genus Streptomyces where it formed a distinct phyletic line with recognized Streptomyces species. Morphological and chemotaxonomic data also supported the affiliation of this isolate to the genus Streptomyces. Strain DUT 180(T) was found to exhibit highest sequence similarities of 99.52 and 99.36 % to Streptomyces halophytocola KLBMP 1284(T) and Streptomyces sulphureus NRRL B-1627(T), respectively. However, strain DUT 180(T) could be distinguished from these two closest neighbours by a range of phenotypic properties. The DNA-DNA hybridization analyses between strain DUT 180(T) and the type strains of the phylogenetic neighbours revealed 54.8 ± 1.4 and 52.4 ± 2.8 % relatedness. Based on the phenotypic, chemotaxonomic and phylogenetic evidence, we suggest that the isolate DUT 180(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces xiaopingdaonensis sp. nov. is proposed, with the type strain DUT 180(T) (= KCTC 29679(T) = CGMCC 4.7208(T)).