Chen Chao, Feng Wei-Wei, Qin Sheng, Zhao Xin-Qing
School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, People's Republic of China.
Antonie Van Leeuwenhoek. 2015 Feb;107(2):511-8. doi: 10.1007/s10482-014-0347-5. Epub 2014 Dec 9.
A novel streptomycete, designated as strain DUT 180(T), was isolated from a marine sediment sample collected from a sea cucumber farm in Dalian, northeast China. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DUT 180(T) is phylogenetically affiliated to the genus Streptomyces where it formed a distinct phyletic line with recognized Streptomyces species. Morphological and chemotaxonomic data also supported the affiliation of this isolate to the genus Streptomyces. Strain DUT 180(T) was found to exhibit highest sequence similarities of 99.52 and 99.36 % to Streptomyces halophytocola KLBMP 1284(T) and Streptomyces sulphureus NRRL B-1627(T), respectively. However, strain DUT 180(T) could be distinguished from these two closest neighbours by a range of phenotypic properties. The DNA-DNA hybridization analyses between strain DUT 180(T) and the type strains of the phylogenetic neighbours revealed 54.8 ± 1.4 and 52.4 ± 2.8 % relatedness. Based on the phenotypic, chemotaxonomic and phylogenetic evidence, we suggest that the isolate DUT 180(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces xiaopingdaonensis sp. nov. is proposed, with the type strain DUT 180(T) (= KCTC 29679(T) = CGMCC 4.7208(T)).
从中国东北大连海参养殖场采集的海洋沉积物样本中分离出一株新型链霉菌，命名为菌株DUT 180(T)。基于16S rRNA基因序列的系统发育分析表明，菌株DUT 180(T)在系统发育上隶属于链霉菌属，在该属中它与已确认的链霉菌物种形成了一个独特的进化分支。形态学和化学分类学数据也支持该分离株隶属于链霉菌属。发现菌株DUT 180(T)与盐生植物链霉菌KLBMP 1284(T)和硫黄链霉菌NRRL B - 1627(T)的序列相似性最高，分别为99.52%和99.36%。然而，菌株DUT 180(T)可通过一系列表型特征与这两个最接近的邻居区分开来。菌株DUT 180(T)与系统发育邻居的模式菌株之间的DNA - DNA杂交分析显示，相关性分别为54.8±1.4%和52.4±2.8%。基于表型、化学分类学和系统发育证据，我们建议分离株DUT 180(T)代表链霉菌属的一个新物种，为此提出新名称小平岛链霉菌（Streptomyces xiaopingdaonensis sp. nov.），模式菌株为DUT 180(T)（= KCTC 29679(T) = CGMCC 4.7208(T)）。
