Shi Jianli, Xu Shaojian, Fu Fang, Cong Xiaoyan, Yuan Xiaoyuan, Peng Zhe, Wu Jiaqiang, Sun Wenbo, Du Yijun, Li Jun, Huang Baohua, Wang Jinbao
College of Life Sciences, Key Laboratory of Animal Resistance of Shandong Province, Shandong Normal University, Jinan, 250014, China,
Arch Virol. 2015 Feb;160(2):465-8. doi: 10.1007/s00705-014-2300-3. Epub 2014 Dec 7.
Porcine circovirus type 2 (PCV2), the essential causative agent of postweaning multisystemic wasting syndrome (PMWS), can be divided into distinct genotypes. Thirty-two PCV2 isolates obtained made from pigs in Shandong Province between 2005 and 2013. Complete genome sequences were obtained in three replicates for each virus isolate, and the sequences were submitted to the NCBI database. The ORF1-encoded amino acid sequences had 98.4 %-100 % identity among the 32 isolates, and there were no significant differences among the three potential glycosylation positions: aa 23-25 (NPS), aa 256-258 (NQT) and aa 286-288 (NAT). The amino acid sequences of ORF2 had 88 %-100 % identity among the 32 isolates and the potential glycosylation position in the cap protein, aa 143-145 (NYS), had no variation. Phylogenetic analysis revealed that the PCV2b/1C genetic lineage was prevalent in swine populations in Shandong Province. It also suggested that selection pressure has made the PCV2 isolates more genetically distant from current vaccine strains.
猪圆环病毒2型（PCV2）是断奶后多系统消耗综合征（PMWS）的主要致病原，可分为不同的基因型。从2005年至2013年期间山东省的猪身上获得了32株PCV2分离株。对每个病毒分离株进行三次重复测序以获得完整的基因组序列，并将序列提交至NCBI数据库。32个分离株中，ORF1编码的氨基酸序列具有98.4%-100%的同一性，并且在三个潜在糖基化位点（第23-25位氨基酸（NPS）、第256-258位氨基酸（NQT）和第286-288位氨基酸（NAT））之间没有显著差异。32个分离株中，ORF2的氨基酸序列具有88%-100%的同一性，并且衣壳蛋白中的潜在糖基化位点（第143-145位氨基酸（NYS））没有变异。系统发育分析表明，PCV2b/1C遗传谱系在山东省猪群中流行。这也表明选择压力使PCV2分离株在基因上与当前疫苗株的距离更远。
