来自智利患有 PMWS 的猪的猪圆环病毒 2 型的遗传分析显示基因间重组。
Genetic analysis of porcine circovirus type 2 from pigs affected with PMWS in Chile reveals intergenotypic recombination.
机构信息
Departamento de Medicina Preventiva, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, Chile.
Sección Virología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
出版信息
Virol J. 2017 Oct 4;14(1):191. doi: 10.1186/s12985-017-0850-1.
BACKGROUND
Porcine circovirus type 2 (PCV2) is a very small, non-enveloped and icosahedral virus, with circular single stranded DNA genome. This virus is the most ubiquitous and persistent pathogen currently affecting the swine industry worldwide. PCV2 has been implicated as the major causative agent of postweaning multisystemic wasting syndrome (PMWS), a disease which is characterized by severe immunosuppressive effects in the porcine host. Worldwide PCV2 isolates have been classified into four different genotypes, PCV2a, PCV2b, PCV2c and PCVd. The goal of this work was to conduct the first phylogenetic analysis of PCV2 in Chile.
METHODS
PCV2 partial ORF2 sequences (462 nt) obtained from 29 clinical cases of PMWS in 22 Chilean intensive swine farms, covering over the 90% of the local pork-production, were analyzed.
RESULTS
14% and 52% of sequences belonged to the genotypes PCV2a and PCV2b, respectively. Surprisingly, 34% of sequences were PCV2a/PCV2d recombinant viruses.
CONCLUSIONS
Our findings suggested that a novel cluster of Chilean sequences emerged resulting from intergenotypic recombination between PCV2a and PCV2d.
背景
猪圆环病毒 2 型（PCV2）是一种非常小的、无包膜的二十面体病毒，具有圆形单链 DNA 基因组。该病毒是目前全球范围内影响养猪业的最普遍和持续存在的病原体。PCV2 被认为是断奶后多系统衰竭综合征（PMWS）的主要致病因子，该疾病的特征是猪宿主严重的免疫抑制作用。全球范围内的 PCV2 分离株已被分为四个不同的基因型，即 PCV2a、PCV2b、PCV2c 和 PCVd。本研究的目的是对智利的 PCV2 进行首次系统进化分析。
方法
从 22 个智利集约化养猪场的 29 个 PMWS 临床病例中获得了 PCV2 部分 ORF2 序列（462nt），覆盖了当地 90%的猪肉生产。
结果
14%和 52%的序列分别属于基因型 PCV2a 和 PCV2b。令人惊讶的是，34%的序列是 PCV2a/PCV2d 重组病毒。
结论
我们的研究结果表明，一种新的智利序列簇是由 PCV2a 和 PCV2d 之间的基因间重组产生的。
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