Kweon Chang-Hee, Nguyen Lien Thi Kim, Yoo Mi-Sun, Kang Seung-Won
Animal and Plant Quarantine Agency, Anyang 430-757, Republic of Korea.
Institute of Genome Research, Vietnam Academy of Science and Technology, Viet Nam.
Gene. 2015 Sep 15;569(2):308-12. doi: 10.1016/j.gene.2015.04.055. Epub 2015 Apr 23.
Porcine circovirus type 2 (PCV2) is the causative agent of post-weaning multisystemic wasting syndrome (PMWS) in swine. Here, a phylogenetic tree was constructed using PCV2 nucleotide sequences derived from the bone marrow of Korean boar and previously reported PCV2 sequences isolated from various countries. PCV2 from Korean boar bone marrow (KC188796) was classified into the group containing PCV2a-Canada and other PCV2 strain from Korea. While the ORF1 region of the PCV2 genome was highly conserved, ORF2 (the capsid protein coding region) was relatively variable. The nucleotide sequences for bone marrow-derived PCV2 were 93.4-99.0% homologous to the other reference sequences. The deduced amino acid sequences for the ORF1 and ORF2 coding regions were 97.4-99.3% and 84.5-97.4% homologous with the other reference strains, respectively, indicating that KC188796 did not differ markedly from the other PCV2 strains. Phylogenetic analysis demonstrated that bone marrow-derived PCV2 was highly similar to PCV2a from Canada and may be related to persistent PCV2 infections in swine.
猪圆环病毒2型（PCV2）是猪断奶后多系统消耗综合征（PMWS）的病原体。在此，利用来自韩国公猪骨髓的PCV2核苷酸序列以及先前报道的从各国分离出的PCV2序列构建了系统发育树。来自韩国公猪骨髓的PCV2（KC188796）被归类到包含PCV2a - 加拿大株以及来自韩国的其他PCV2毒株的组中。虽然PCV2基因组的ORF1区域高度保守，但ORF2（衣壳蛋白编码区）相对可变。骨髓来源的PCV2的核苷酸序列与其他参考序列的同源性为93.4 - 99.0%。ORF1和ORF2编码区推导的氨基酸序列与其他参考毒株的同源性分别为97.4 - 99.3%和84.5 - 97.4%，这表明KC188796与其他PCV2毒株没有明显差异。系统发育分析表明，骨髓来源的PCV2与来自加拿大的PCV2a高度相似，可能与猪的PCV2持续感染有关。
