唑来膦酸对关节软骨细胞和滑膜细胞血管内皮生长因子-a表达的影响：一项体外研究。
Effect of zoledronate on the expression of vascular endothelial growth factor-a by articular chondrocytes and synovial cells: an in vitro study.
作者信息
Yi Jin Woong, Lee Woo-Suk, Kim Sang-Bum, Heo Youn-Moo, Chae Dong-Sik
机构信息
Department of Orthopedic Surgery, Konyang University Hospital, Daejeon, Korea.
Department of Orthopedic Surgery, Yonsei University Gangnam Severance Hospital, Seoul, Korea.
出版信息
J Bone Metab. 2014 Nov;21(4):249-55. doi: 10.11005/jbm.2014.21.4.249. Epub 2014 Nov 30.
BACKGROUND
The aim of this in vitro study was to determine the effect of zoledronate, which is frequently used to treat osteoporosis, on osteoarthritis by analyzing zoledronate-induced expression of vascular endothelial growth factor-A (VEGF-A) in chondrocytes and synovial cells.
METHODS
After chondrocytes and synovial cells were separated and cultured, zoledronate was added, and VEGF-A and pigment epithelium-derived factor (PEDF) expression were quantified by real-time polymerase chain reaction and Western blotting.
RESULTS
There was no significant difference in the expression of VEGF-A mRNA in chondrocytes between the zoledronate group and the control group on the 8th day of culture. The expression of both VEGF-A and PEDF mRNA in synovial cells was significantly decreased in the zoledronate group (P<0.05).
CONCLUSIONS
Zoledronate decreases the expression of VEGF-A in synovial cells and may affect the development and progression of osteoarthritis.
背景
本体外研究的目的是通过分析唑来膦酸诱导软骨细胞和滑膜细胞中血管内皮生长因子-A（VEGF-A）的表达，来确定常用于治疗骨质疏松症的唑来膦酸对骨关节炎的影响。
方法
分离并培养软骨细胞和滑膜细胞后，加入唑来膦酸，通过实时聚合酶链反应和蛋白质印迹法对VEGF-A和色素上皮衍生因子（PEDF）的表达进行定量。
结果
培养第8天时，唑来膦酸组与对照组软骨细胞中VEGF-A mRNA的表达无显著差异。唑来膦酸组滑膜细胞中VEGF-A和PEDF mRNA的表达均显著降低（P<0.05）。
结论
唑来膦酸可降低滑膜细胞中VEGF-A的表达，并可能影响骨关节炎的发生和发展。
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