Zhang ShanYong, Cao Wei, Wei KuiJie, Liu XiuMing, Xu YuanJin, Yang Chi, Undt Gerhard, Haddad Majd S, Chen WanTao
Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, Collage of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
Br J Oral Maxillofac Surg. 2013 Jan;51(1):69-73. doi: 10.1016/j.bjoms.2012.01.014. Epub 2012 Feb 17.
Our aim was to evaluate the expression of vascular endothelial growth factor receptors (VEGFRs) in the synovium of the temporomandibular joints (TMJ) of rabbits with experimentally induced internal derangement. Internal derangement was experimentally induced in 52 rabbit TMJ, and established on the right side of TMJ while the left side was used as the control. Each joint and its control was evaluated by magnetic resonance imaging (MRI) and endoscopy. The synovial tissues on both sides were harvested after one, two, three, and four weeks. The expression of VEGFRs mRNA was investigated in the experimental joint and its control using real-time polymerase chain reaction (PCR). Internal derangement was successfully confirmed in 45 of the 52 of the experimental joints (87%) on the right side by MRI and endoscopy. In the first and fourth week, the VEGFR-2 mRNA expression was higher in the experimental joints than in the controls (P=0.008 and P=0.02). Meanwhile, the VEGFR-1 mRNA expression was up-regulated in the experimental group compared with the controls during the fourth week (P=0.02). However, we found no significant differences in VEGFR-3 mRNA expression in the two groups during the first and fourth weeks. During the second and third weeks, the mRNA expression of the three receptors did not differ significantly among the groups. Our data have shown increased expression of VEGFR-1 and VEGFR-2 mRNA in the synovium of rabbit TMJ with internal derangement, which indicates that VEGFR-1 and VEGFR-2 may have important roles in the processes of internal derangement and formation of adhesions.
我们的目的是评估实验性诱导关节内紊乱的兔颞下颌关节（TMJ）滑膜中血管内皮生长因子受体（VEGFRs）的表达。对52个兔TMJ进行实验性关节内紊乱诱导，并在TMJ右侧建立模型，左侧作为对照。通过磁共振成像（MRI）和内窥镜检查对每个关节及其对照进行评估。在1、2、3和4周后采集两侧的滑膜组织。使用实时聚合酶链反应（PCR）研究实验关节及其对照中VEGFRs mRNA的表达。通过MRI和内窥镜检查成功证实52个实验关节中有45个（87%）右侧出现关节内紊乱。在第1周和第4周，实验关节中VEGFR-2 mRNA表达高于对照（P=0.008和P=0.02）。同时，在第4周时，实验组中VEGFR-1 mRNA表达与对照相比上调（P=0.02）。然而，我们发现在第1周和第4周两组中VEGFR-3 mRNA表达无显著差异。在第2周和第3周，三组中三种受体的mRNA表达无显著差异。我们的数据表明，关节内紊乱的兔TMJ滑膜中VEGFR-1和VEGFR-2 mRNA表达增加，这表明VEGFR-1和VEGFR-2可能在关节内紊乱和粘连形成过程中起重要作用。
