Wen Can, Lv Jiao-Feng, Wang Ling, Zhu Wei-Feng, Wan Fu-Sheng, Wang Xiao-Zhong
1 Department of Clinical Laboratory Medicine, The Second Affiliated Hospital of Nanchang University , Nanchang, China .
Genet Test Mol Biomarkers. 2015 Jan;19(1):24-9. doi: 10.1089/gtmb.2014.0213.
The methylene tetrahydrofolate reductase (MTHFR) C677T polymorphism is associated with hypertension in certain populations. This study investigated the relationship between the MTHFR polymorphism and hypertension and correlated blood lipid indexes, including homocysteine (HCY), lipoprotein (a) [Lp (a)], high-density lipoprotein (HDL), low-density lipoprotein (LDL), apolipoprotein A I (Apo AI), Apo B, glucose (GLU), total cholesterol (TC), and triglyceride (TG), in a Chinese population.
A total of 174 patients with hypertension and 634 healthy control individuals from Jiangxi Province were recruited between June 2012 and September 2012 for genotyping of the MTHFR C677T polymorphism using polymerase chain reaction-restriction fragment length polymorphism. Biochemical parameters were also assessed in these subjects and statistically compared to the MTHFR C677T polymorphism and the risk for hypertension.
HCY and Lp (a) levels were significantly higher in subjects with a MTHFR 677TT genotype than in those with a CC/CT genotype, independent of hypertension. The frequency of the TT genotype and the T allele in hypertension patients was significantly higher than in the healthy controls. Furthermore, in the male hypertension patient group, the average levels of HCY, HDL, Apo AI, and TC were significantly different from those in female hypertension patients (pHCY=0.001, pHDL=0.004, pApo AI<0.001, pTC=0.012). In the male control group, the average levels of HCY, HDL, Apo AI, GLU, and TC were significantly different from those of female controls (pHCY<0.001, pHDL<0.001, pApo AI<0.001, pGLU=0.001, and pTC=0.004).
Our data demonstrate that the MTHFR C677T polymorphism is positively correlated with an increased risk of hypertension through an increase in HCY levels. The blood lipid correlative index was different between male and female hypertension patients and controls.
亚甲基四氢叶酸还原酶（MTHFR）C677T基因多态性在某些人群中与高血压相关。本研究调查了中国人群中MTHFR基因多态性与高血压及相关血脂指标之间的关系，这些血脂指标包括同型半胱氨酸（HCY）、脂蛋白（a）[Lp（a）]、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）、载脂蛋白A I（Apo AI）、Apo B、葡萄糖（GLU）、总胆固醇（TC）和甘油三酯（TG）。
2012年6月至2012年9月期间，共招募了来自江西省的174例高血压患者和634例健康对照个体，采用聚合酶链反应-限制性片段长度多态性方法对MTHFR C677T基因多态性进行基因分型。还对这些受试者的生化参数进行了评估，并与MTHFR C677T基因多态性及高血压风险进行了统计学比较。
MTHFR 677TT基因型受试者的HCY和Lp（a）水平显著高于CC/CT基因型受试者，与高血压无关。高血压患者中TT基因型和T等位基因的频率显著高于健康对照。此外，在男性高血压患者组中，HCY、HDL、Apo AI和TC的平均水平与女性高血压患者显著不同（pHCY = 0.001，pHDL = 0.004，pApo AI < 0.001，pTC = 0.012）。在男性对照组中，HCY、HDL、Apo AI、GLU和TC的平均水平与女性对照组显著不同（pHCY < 0.001，pHDL < 0.001，pApo AI < 0.001，pGLU = 0.001，pTC = 0.004）。
我们的数据表明，MTHFR C677T基因多态性通过增加HCY水平与高血压风险增加呈正相关。男性和女性高血压患者及对照之间的血脂相关指标存在差异。
