Bayramoglu Aysegul, Urhan Kucuk Meral, Guler Halıl Ibrahim, Abaci Okay, Kucukkaya Yunus, Colak Ertugrul
Department of Biology, Science and Art Faculty, Artvin Coruh University, 08000, Artvin, Turkey,
Cytotechnology. 2015 Jan;67(1):115-22. doi: 10.1007/s10616-013-9665-0. Epub 2013 Nov 21.
The current study was conducted to determine whether there is a relation between hypertension and two different polymorphisms, including C1562T of the Matrix metalloproteinase-9 (MMP-9) gene and C677T of the methylenetetrahydrofolate reductase (MTHFR) gene. Genomic DNA obtained from 224 persons (125 patients with hypertension and 99 healthy controls) were used in the study. Polymorphisms were determined by using polymerase chain reaction-restriction fragment length polymorphism and electrophoresis. The results were statistically analyzed and were found to be statistically significant. The frequencies of the C1562T genotypes were found to be, in controls CC 75.8 % and CT 24.2 % and in patients CC 71.2 %, and CT 28.8 %. The frequencies of C677T genotype were found to be, in controls CC 56.6 %, CT 38.4 and TT 5.1 % in controls and in patients CC 52 %, CT 30.4 % and TT 17.6 %. In conclusion, we may suggest that there is no relation between the essential hypertension and C1562T polymorphism of MMP-9 gene; on the other hand C677T polymorphism (genotype TT) of MTHFR gene can be regarded as a genetic indicator for the development of essential hypertension.
本研究旨在确定高血压与两种不同的基因多态性之间是否存在关联，这两种多态性包括基质金属蛋白酶-9（MMP-9）基因的C1562T和亚甲基四氢叶酸还原酶（MTHFR）基因的C677T。本研究使用了从224人（125例高血压患者和99例健康对照）获取的基因组DNA。通过聚合酶链反应-限制性片段长度多态性和电泳来确定多态性。对结果进行统计学分析，发现具有统计学意义。C1562T基因型的频率在对照组中CC为75.8%，CT为24.2%；在患者中CC为71.2%，CT为28.8%。C677T基因型的频率在对照组中CC为56.6%，CT为38.4%，TT为5.1%；在患者中CC为52%，CT为30.4%，TT为17.6%。总之，我们可能认为原发性高血压与MMP-9基因的C1562T多态性之间没有关联；另一方面，MTHFR基因的C677T多态性（基因型TT）可被视为原发性高血压发生的一个遗传指标。
