Zhang S M, Wu M, Chen H, Zhang X
Institute of Hepatobiliary Surgery, Changhai Hospital, Shanghai, People's Republic of China.
Histochemistry. 1989;92(2):171-5. doi: 10.1007/BF00490238.
Receptors of 12 lectins in 25 cases of human hepatocellular carcinomas (HCC) were histochemically investigated by avidin-biotin-peroxidase complex (ABC) method. Liver tissues of five cirrhotic patients and five normal subjects were used as controls. SJA receptor was absent both in HCC and controls, while LCA and PSA receptors were present in all tissues studied here. Receptors of DBA, PHA, PNA, UEAI and SBA which did not bind to normal, cirrhotic and pericarcinomatous liver tissues had the positive rates of 4%, 44%, 16%, 4% and 12% in HCC, respectively. Four lectins which strongly bound to the non-cancer liver tissues had their receptors in 96% (ConA, WGA, RCAI) and 36% (BSAI) of HCC. The pretreatment of tissue sections with neuraminidase abolished most of WGA receptors and exposed some PNA binding sites. There were many differences in lectin distribution between HCC and noncancer liver tissues. The changes of glycoconjugates in HCC were discussed.
采用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）法，对25例人类肝细胞癌（HCC）中12种凝集素的受体进行了组织化学研究。以5例肝硬化患者和5例正常受试者的肝组织作为对照。HCC和对照中均不存在荆豆凝集素（SJA）受体，而刀豆球蛋白A（LCA）和花生凝集素（PSA）受体在所有研究组织中均有存在。不能与正常、肝硬化和癌旁肝组织结合的双花扁豆凝集素（DBA）、植物血凝素（PHA）、花生凝集素（PNA）、荆豆凝集素I（UEAI）和大豆凝集素（SBA）的受体在HCC中的阳性率分别为4%、44%、16%、4%和12%。能与非癌肝组织强烈结合的4种凝集素，其受体在96%的HCC中存在（刀豆球蛋白A、小麦胚凝集素、蓖麻凝集素I），在36%的HCC中存在（大豆凝集素I）。用神经氨酸酶对组织切片进行预处理可消除大部分小麦胚凝集素受体，并暴露一些花生凝集素结合位点。HCC与非癌肝组织之间的凝集素分布存在许多差异。对HCC中糖缀合物的变化进行了讨论。
