高斯光束在大鼠脑微血管双光子荧光成像中的应用
Gaussian beam in two-photon fluorescence imaging of rat brain microvessel.
作者信息
Shi Lingyan, Rodríguez-Contreras Adrián, Alfano Robert R
机构信息
The City College of New York, Department of Biomedical Engineering, 160 Convent Avenue, New York, New York 10031, United StatesbThe City College of New York, Department of Biology, 160 Convent Avenue, New York, New York 10031, United StatescThe City Colle.
The City College of New York, Department of Biology, 160 Convent Avenue, New York, New York 10031, United StatescThe City College of New York, Institute for Ultrafast Spectroscopy and Lasers and Departments of Electrical Engineering and Physics, 160 Conve.
出版信息
J Biomed Opt. 2014 Dec;19(12):126006. doi: 10.1117/1.JBO.19.12.126006.
Abstract
The critical optical properties of a Gaussian laser beam in two-photon or multiphoton fluorescence imaging, including the beam spot size, depth of focus, and intensity profile, are investigated for spatially locating nanoscale solutes in and surrounding the microvessels of rat brain.
摘要
研究了高斯激光束在双光子或多光子荧光成像中的关键光学特性，包括光斑尺寸、焦深和强度分布，以在空间上定位大鼠脑微血管内及周围的纳米级溶质。
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