通过完整颅骨对小鼠大脑结构和功能进行三光子成像。
Three-photon imaging of mouse brain structure and function through the intact skull.
机构信息
School of Applied and Engineering Physics, Cornell University, Ithaca, NY, USA.
CNC Program, Stanford University, Stanford, CA, USA.
出版信息
Nat Methods. 2018 Oct;15(10):789-792. doi: 10.1038/s41592-018-0115-y. Epub 2018 Sep 10.
Optical imaging through the intact mouse skull is challenging because of skull-induced aberrations and scattering. We found that three-photon excitation provided improved optical sectioning compared with that obtained with two-photon excitation, even when we used the same excitation wavelength and imaging system. Here we demonstrate three-photon imaging of vasculature through the adult mouse skull at >500-μm depth, as well as GCaMP6s calcium imaging over weeks in cortical layers 2/3 and 4 in awake mice, with 8.5 frames per second and a field of view spanning hundreds of micrometers.
通过完整的小鼠颅骨进行光学成像是具有挑战性的，因为颅骨会引起像差和散射。我们发现，与双光子激发相比，三光子激发提供了更好的光学切片，即使我们使用相同的激发波长和成像系统。在这里，我们展示了通过成年小鼠颅骨进行的三光子血管成像，深度超过 500μm，以及在清醒小鼠的皮层 2/3 和 4 层中进行的 GCaMP6s 钙成像，帧率为每秒 8.5 帧，视场跨越数百微米。
Zotero 插件