Tn5中心区域核苷酸序列的完成证实了三个抗性基因的存在。
Completion of the nucleotide sequence of the central region of Tn5 confirms the presence of three resistance genes.
作者信息
Mazodier P, Cossart P, Giraud E, Gasser F
出版信息
Nucleic Acids Res. 1985 Jan 11;13(1):195-205. doi: 10.1093/nar/13.1.195.
Abstract
The DNA sequence of the region located downstream from the kanamycin resistance gene of Tn5 up to the right inverted repeat IS50R has been determined. This completes the determination of the sequence of Tn5 which is 5818 bp long. The 2.7 Kb central region contains three resistance genes: the kanamycin-neomycin resistance gene, a gene coding for resistance to CL990 an antimitotic-antibiotic compound of the bleomycin family and a third gene that confers streptomycin resistance in some bacterial species but is cryptic in E. coli. A Tn5* mutant able to express streptomycin resistance in E. coli was isolated. With this mutant, it was demonstrated that in E. coli the expression of the three resistance genes is coordinated in a single operon.
摘要
已确定了Tn5卡那霉素抗性基因下游直至右侧反向重复序列IS50R区域的DNA序列。这完成了对长度为5818 bp的Tn5序列的测定。2.7 Kb的中央区域包含三个抗性基因：卡那霉素-新霉素抗性基因、一个编码对博来霉素家族抗有丝分裂抗生素化合物CL990抗性的基因以及第三个基因，该基因在某些细菌物种中赋予链霉素抗性，但在大肠杆菌中不表达。分离出了一种能够在大肠杆菌中表达链霉素抗性的Tn5*突变体。利用该突变体证明，在大肠杆菌中，这三个抗性基因的表达在一个单一操纵子中是协调的。
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