Zafar Hina, Kareem Abdul, Sherwani Asif, Mohammad Owais, Ansari Mohammad Azam, Khan Haris M, Khan Tahir Ali
Division of Inorganic Chemistry, Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India.
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India.
J Photochem Photobiol B. 2015 Jan;142:8-19. doi: 10.1016/j.jphotobiol.2014.10.004. Epub 2014 Oct 24.
A condensation reaction between 1,2-diphenylethane-1,2-dione dihydrazone (DPEDDH) and dimethyl or diethyloxalate in methanol resulted in a novel Schiff base octaazamacrocyclic ligand, (L): (6,7,14,15-tetraoxa-2,3,10,11-tetraphenyl-1,4,5,8,9,12,13,16-octaazacyclohexadecane-1,3,9,11-tetraene). Subsequently metal complexes of the type [MLX2] and [CuL]X2; (M=Mn(II), Co(II), Ni(II) and Zn(II); X=Cl or NO3) were synthesized by the reaction of the free macrocyclic ligand (L) with the corresponding metal salts in 1:1 molar ratio. These complexes were characterized on the basis of analytical data, molar conductivity and magnetic susceptibility measurements, ESI-mass, IR, NMR ((1)H and (13)C), EPR and electronic spectral studies. The thermal stability of the complexes was also studied by TGA and DTA analyses. These studies show that all the complexes have octahedral arrangement around the metal ions except copper complexes which are square planar. The ligand and its complexes were screened for their antibacterial activity in vitro against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria and were also studied for their anticancer activity against the human cancer cells lines: HeLa (Human cervical carcinoma), MCF7 (Human breast adenocarcinoma) and Hep3B (Human Hepatocellular carcinoma). The recorded IC50 values for the tested compounds show moderate to good cytotoxicity against these cancer cell lines. The copper complex, [CuL]Cl2, showed excellent antimicrobial activity against tested microorganisms which is almost equivalent to the standard drug ciprofloxacin.
1,2 - 二苯基乙烷 - 1,2 - 二酮二腙（DPEDDH）与草酸二甲酯或草酸二乙酯在甲醇中发生缩合反应，生成了一种新型席夫碱八氮大环配体（L）：（6,7,14,15 - 四氧杂 - 2,3,10,11 - 四苯基 - 1,4,5,8,9,12,13,16 - 八氮杂环十六烷 - 1,3,9,11 - 四烯）。随后，通过游离大环配体（L）与相应金属盐以1:1摩尔比反应，合成了[MLX₂]和[CuL]X₂型金属配合物（M = Mn(II)、Co(II)、Ni(II)和Zn(II)；X = Cl或NO₃）。这些配合物通过分析数据、摩尔电导率和磁化率测量、电喷雾质谱、红外光谱、核磁共振（¹H和¹³C）、电子顺磁共振和电子光谱研究进行了表征。还通过热重分析（TGA）和差示热分析（DTA）研究了配合物的热稳定性。这些研究表明，除了呈平面正方形的铜配合物外，所有配合物在金属离子周围均具有八面体构型。对配体及其配合物进行了体外抗革兰氏阳性菌（金黄色葡萄球菌）和革兰氏阴性菌（大肠杆菌）的抗菌活性筛选，并研究了它们对人癌细胞系HeLa（人宫颈癌）、MCF7（人乳腺腺癌）和Hep3B（人肝细胞癌）的抗癌活性。所测化合物记录的半数抑制浓度（IC₅₀）值显示出对这些癌细胞系具有中度至良好的细胞毒性。铜配合物[CuL]Cl₂对受试微生物表现出优异的抗菌活性，几乎等同于标准药物环丙沙星。
