PG and Research Department of Chemistry, Bishop Heber College, Tiruchirappalli, Tamilnadu, 620017, India.
School of Chemistry, Bharathidasan University, Tiruchirappalli, Tamilnadu, 620024, India.
J Fluoresc. 2017 Jan;27(1):135-150. doi: 10.1007/s10895-016-1941-x. Epub 2016 Oct 1.
Novel bioactive complexes of Co(II), Cu(II), Ni(II) and Zn(II) metal ions with Schiff base ligand derived from histidine and 1,3-indandione were synthesized and thoroughly characterized by various analytical and spectral techniques. The biological investigations were carried out to examine the efficiency of the binding interaction of all the complexes with calf thymus DNA (CT-DNA). The binding properties were studied and evaluated quantitatively by K and K values using UV-visible, fluorescence spectroscopy and voltammetric techniques. The experimental results revealed that the mode of binding of all the complexes with CT-DNA is via intercalation. It is further verified by viscosity measurements and thermal denaturation experiments. From the results of the cleavage study with pUC19 DNA it is inferred that all the complexes possess excellent cleaving ability. The present investigation proved that the binding interaction of all the complexes are significantly strong and the order of binding strength of the complexes is [Ni(L)] (K = 3.11 × 10 M) > [Co(L)] (K = 2.89 × 10 M) > [Cu(L)] (K = 2.64 × 10 M) > [Zn(L)] (K = 2.41 × 10 M). The complexes were also screened for antibacterial and anticandidal activity. The in vitro cytotoxicity of the ligand and complexes on the NIH/3 T3 mouse fibroblast cell lines were examined using CellTiter-Blue® (CTB) Cell viability assay, which unveiled that all the complexes exhibit more potent activities against NIH/3 T3 cells. Among all the complexes [Zn(L)] complex showed the maximum efficiency.
新型 Co(II)、Cu(II)、Ni(II) 和 Zn(II) 金属离子与组氨酸和 1,3-茚二酮衍生的希夫碱配体的生物活性配合物被合成，并通过各种分析和光谱技术进行了彻底的表征。进行了生物研究，以检查所有配合物与小牛胸腺 DNA（CT-DNA）结合相互作用的效率。使用紫外-可见光谱、荧光光谱和伏安技术通过 K 和 K 值研究和定量评估了结合特性。实验结果表明，所有配合物与 CT-DNA 的结合模式是嵌入。通过粘度测量和热变性实验进一步验证。从 pUC19 DNA 的切割研究结果推断，所有配合物都具有优异的切割能力。目前的研究证明，所有配合物的结合相互作用都非常强，配合物的结合强度顺序为 [Ni(L)]（K=3.11×10^M）> [Co(L)]（K=2.89×10^M）> [Cu(L)]（K=2.64×10^M）> [Zn(L)]（K=2.41×10^M）。还对这些配合物进行了抗菌和抗真菌活性筛选。使用 CellTiter-Blue®（CTB）细胞活力测定法，在 NIH/3T3 小鼠成纤维细胞系上检查了配体和配合物的体外细胞毒性，结果表明所有配合物对 NIH/3T3 细胞均表现出更强的活性。在所有配合物中，[Zn(L)]配合物表现出最大的效率。
