Dhayabaran V Violet, Prakash T Daniel
PG and Research Department of Chemistry, Bishop Heber College (Autonomous), Tiruchirappalli, Tamilnadu, -620017, India.
J Fluoresc. 2016 Sep;26(5):1825-37. doi: 10.1007/s10895-016-1874-4. Epub 2016 Jul 15.
Synthesis of new metallic complex of cobalt and zinc with amino acid-nucleobase hybrid ligand have been achieved by simple chemical reaction of metal salt with amino acid L-histidine and nucleobase adenine as ligands. Various physicochemical techniques such as elemental analysis, conductometric measurements, FT-IR, UV-visible, (1)H & (13)C NMR, mass spectroscopy and magnetic measurements were employed to characterize the complexes. The results confirmed the formation of the ligand and the complex. The interaction of the complex with calf thymus DNA (CT-DNA) has been carried out using UV-visible titration, fluorescence spectroscopy, cyclic voltammetry and viscosity measurements. The intrinsic binding constant (Kb) and Stern-Volmer constant (Ksv) of the complexes have been calculated. The cleavage activity of the ligand and the complexes with pBR322 DNA was further confirmed by gel electrophoretic technique. The pharmacological activity of the ligand and the complexes was investigated by antioxidant, antimicrobial and cytotoxic activity, and they show promising pharmacological effect. The results of the molecular docking studies of the ligand and the complexes reinforce all the above facts.
通过金属盐与氨基酸L-组氨酸和核碱基腺嘌呤作为配体的简单化学反应，合成了钴和锌与氨基酸-核碱基杂化配体的新型金属配合物。采用了各种物理化学技术，如元素分析、电导测量、傅里叶变换红外光谱（FT-IR）、紫外可见光谱、（1）H和（13）C核磁共振、质谱和磁性测量来表征配合物。结果证实了配体和配合物的形成。使用紫外可见滴定、荧光光谱、循环伏安法和粘度测量研究了配合物与小牛胸腺DNA（CT-DNA）的相互作用。计算了配合物的固有结合常数（Kb）和斯特恩-沃尔默常数（Ksv）。通过凝胶电泳技术进一步证实了配体和配合物对pBR322 DNA的切割活性。通过抗氧化、抗菌和细胞毒性活性研究了配体和配合物的药理活性，它们显示出有前景的药理作用。配体和配合物的分子对接研究结果强化了上述所有事实。
