Kessler Sharon A, Lindner Heike, Jones Daniel S, Grossniklaus Ueli
Institute of Plant Biology & Zürich-Basel Plant Science Center, University of Zürich, Zürich, Switzerland Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, USA
Institute of Plant Biology & Zürich-Basel Plant Science Center, University of Zürich, Zürich, Switzerland.
EMBO Rep. 2015 Jan;16(1):107-15. doi: 10.15252/embr.201438801. Epub 2014 Dec 9.
The Catharanthus roseus Receptor-Like Kinase 1-like (CrRLK1L) family of 17 receptor-like kinases (RLKs) has been implicated in a variety of signaling pathways in Arabidopsis, ranging from pollen tube (PT) reception and tip growth to hormonal responses. The extracellular domains of these RLKs have malectin-like domains predicted to bind carbohydrate moieties. Domain swap analysis showed that the extracellular domains of the three members analyzed (FER, ANX1, HERK1) are not interchangeable, suggesting distinct upstream components, such as ligands and/or co-factors. In contrast, their intercellular domains are functionally equivalent for PT reception, indicating that they have common downstream targets in their signaling pathways. The kinase domain is necessary for FER function, but kinase activity itself is not, indicating that other kinases may be involved in signal transduction during PT reception.
长春花类受体激酶1样（CrRLK1L）家族由17种类受体激酶（RLK）组成，参与了拟南芥中的多种信号通路，从花粉管（PT）识别和顶端生长到激素反应。这些RLK的胞外结构域具有预测可结合碳水化合物部分的类苹果酸结合蛋白结构域。结构域交换分析表明，所分析的三个成员（FER、ANX1、HERK1）的胞外结构域不可互换，这表明存在不同的上游成分，如配体和/或辅助因子。相反，它们的胞内结构域在PT识别功能上是等效的，这表明它们在信号通路中有共同的下游靶点。激酶结构域对于FER功能是必需的，但激酶活性本身并非必需，这表明在PT识别过程中可能有其他激酶参与信号转导。
