马铃薯（Solanum tuberosum L.）中类受体激酶 CrRLK1 基因家族的全基因组鉴定、表达分析及其在病原体相关分子模式触发免疫中的作用

Genome-Wide Identification and Expression Analysis of CrRLK1-like Gene Family in Potatoes ( L.) and Its Role in PAMP-Triggered Immunity.

作者信息

Bao Yazhou, Zhao Ru, Hu Sixian, Li Xiaoli, Wang Like, Wang Ji, Ji Junbin, Wang Weiduo, Zhu Changqing, Chen Jiajia, Ben Ailing, Peng Jinfeng, Liu Tingli

机构信息

The Nanjing Engineering Research Center for Peanut Genetic Engineering Breeding and Industrialization, School of Food Science, Nanjing Xiaozhuang University, Nanjing 211171, China.

College of Landscape Architecture, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212400, China.

出版信息

Genes (Basel). 2025 Mar 4;16(3):308. doi: 10.3390/genes16030308.

DOI:10.3390/genes16030308

PMID:40149459

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11942165/

Abstract

BACKGROUND

The receptor-like kinase 1-like (CrRLK1L) subfamily, a specialized group within receptor-like kinases (RLKs), was initially identified in cell cultures. CrRLK1L plays an important role in the growth, development and stress response of plants. Although CrRLK1L genes have been characterized across multiple plant species, their biological and genetic functions in potatoes () remains poorly elucidated.

METHODS

a genome-wide investigation, phylogenetic analysis, chromosome localization, exon-intron structure, conserved motifs, stress-responsive -elements, tissue-specific expression patterns, and their effects on pathogen associated molecular patterns (PAMPs) induced reactive oxygen species (ROS) production were analyzed.

RESULTS

A total of 29 CrRLK1L genes were identified in the genome, unevenly distributed across 10 chromosomes and divided into three groups. Tissue-specific expression analysis revealed seven genes highly expressed in all tissues, while CrRLK1L13 was specific to stamens and flowers. Under stress conditions (mannitol, salt, hormone, and heat), StCrRLK1L genes exhibited diverse expression patterns. Functional characterization in identified seven ROS suppressors and four ROS enhancers, implicating their roles in PAMP-triggered immunity.

CONCLUSIONS

This study provides valuable insights into the StCrRLK1L gene family, enhancing our understanding of its functions, particularly in plant innate immunity.

摘要

背景

类受体激酶1样（CrRLK1L）亚家族是类受体激酶（RLK）中的一个特殊群体，最初在细胞培养物中被鉴定出来。CrRLK1L在植物的生长、发育和应激反应中起重要作用。尽管CrRLK1L基因已在多种植物物种中得到表征，但其在马铃薯中的生物学和遗传功能仍未得到充分阐明。

方法

进行了全基因组研究、系统发育分析、染色体定位、外显子-内含子结构、保守基序、应激反应元件、组织特异性表达模式分析，以及它们对病原体相关分子模式（PAMP）诱导的活性氧（ROS）产生的影响分析。

结果

在马铃薯基因组中总共鉴定出29个CrRLK1L基因，它们不均匀地分布在10条染色体上，并分为三组。组织特异性表达分析显示，有7个基因在所有组织中高表达，而CrRLK1L13对雄蕊和花具有特异性。在应激条件（甘露醇、盐、激素和热）下，StCrRLK1L基因表现出不同的表达模式。在马铃薯中的功能表征鉴定出7个ROS抑制因子和4个ROS增强因子，表明它们在PAMP触发的免疫中发挥作用。